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Species-specific alternative splicing leads to unique expression of sno-lncRNAs

文献类型：期刊论文

作者Zhang XO1; Yin QF2; Wang HB3; Zhang Y2; Chen T2; Zheng P4; Lu XH3; Chen LL[*]2; Yang L[*]1
刊名BMC GENOMICS
出版日期2014
卷号15期号:X页码:e287
关键词lncRNA sno-lncRNA Alternative splicing PWS
通讯作者linglingchen@sibcb.ac.cn ; liyang@picb.ac.cn
合作状况其它
英文摘要Background: Intron-derived long noncoding RNAs with snoRNA ends (sno-lncRNAs) are highly expressed from the imprinted Prader-Willi syndrome (PWS) region on human chromosome 15. However, sno-lncRNAs from other regions of the human genome or from other genomes have not yet been documented. 

Results: By exploring non-polyadenylated transcriptomes from human, rhesus and mouse, we have systematically annotated sno-lncRNAs expressed in all three species. In total, using available data from a limited set of cell lines, 19 sno-lncRNAs have been identified with tissue-and species-specific expression patterns. Although primary sequence analysis revealed that snoRNAs themselves are conserved from human to mouse, sno-lncRNAs are not. PWS region sno-lncRNAs are highly expressed in human and rhesus monkey, but are undetectable in mouse. Importantly, the absence of PWS region sno-lncRNAs in mouse suggested a possible reason why current mouse models fail to fully recapitulate pathological features of human PWS. In addition, a RPL13A region sno-lncRNA was specifically revealed in mouse embryonic stem cells, and its snoRNA ends were reported to influence lipid metabolism. Interestingly, the RPL13A region sno-lncRNA is barely detectable in human. We further demonstrated that the formation of sno-lncRNAs is often associated with alternative splicing of exons within their parent genes, and species-specific alternative splicing leads to unique expression pattern of sno-lncRNAs in different animals. 

Conclusions: Comparative transcriptomes of non-polyadenylated RNAs among human, rhesus and mouse revealed that the expression of sno-lncRNAs is species-specific and that their processing is closely linked to alternative splicing of their parent genes. This study thus further demonstrates a complex regulatory network of coding and noncoding parts of the mammalian genome.
收录类别SCI
资助信息This work was supported by grants 2014CB964800 and 2014CB910600 from MOST, XDA01010206 and 2012OHTP08 from CAS, 31322018, 31271376 and 31271390 from NSFC.
语种英语
WOS记录号WOS:000335406900001
公开日期2014-10-15
源URL[http://159.226.149.42:8088/handle/152453/8053]  
专题昆明动物研究所_哺乳动物胚胎发育
昆明动物研究所_遗传资源与进化国家重点实验室
作者单位1.Key Laboratory of Computational Biology, CAS-MPG Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
2.State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
3.Department of Orthopedic Surgery, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China
4.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China
推荐引用方式
GB/T 7714		 Zhang XO,Yin QF,Wang HB,et al. Species-specific alternative splicing leads to unique expression of sno-lncRNAs[J]. BMC GENOMICS,2014,15(X):e287.
APA Zhang XO.,Yin QF.,Wang HB.,Zhang Y.,Chen T.,...&Yang L[*].(2014).Species-specific alternative splicing leads to unique expression of sno-lncRNAs.BMC GENOMICS,15(X),e287.
MLA Zhang XO,et al."Species-specific alternative splicing leads to unique expression of sno-lncRNAs".BMC GENOMICS 15.X(2014):e287.

入库方式： OAI收割

来源：昆明动物研究所

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