Transcriptome analyses of rhesus monkey pre-implantation embryos reveal a reduced capacity for DNA double strand break repair in primate oocytes and early embryos
文献类型：期刊论文
| 作者 | Wang XY1,2,3,4; Liu DH3,5; He XC1,2,6; Han JD[*]5; Zheng P[*]1,2,6; He DJ1,2,3,4; Suo SB3,5; Xia X3,5 |
| 刊名 | GENOME RESEARCH
 |
| 出版日期 | 2017 |
| 卷号 | 27期号:X页码:567–579 |
| 通讯作者 | jdhan@picb.ac.cn |
| 英文摘要 | Pre-implantation embryogenesis encompasses several critical events including genome reprogramming, zygotic genome activation (ZGA), and cell fate commitment, most of which remain mechanistically unclear in primates. In addition, primates display a high rate of embryo wastage without any clear molecular basis. Understanding the factors involved in genome reprogramming and ZGA will help the generation of induced pluripotent stem cells with high efficiency. Moreover, explaining the molecular basis responsible for embryo wastage in primates will greatly expand our knowledge of species evolution. Here, we have carried out time-series RNA-seq studies in single and pooled rhesus monkey oocytes and pre-implantation embryos at representative developmental stages. By comparing to human and mouse data, we found that the transcriptome dynamics of monkey oocytes and embryos were very similar to those of human, but very different from those of mouse. We identified several classes of maternal and zygotic genes, whose expression peaks were highly correlated with the time frames of genome reprogramming, ZGA and cell fate commitment, respectively. Importantly, comparison of the ZGA-related network modules among the three species revealed a looser surveillance of genomic instability in primate oocytes and embryos than in rodents, particularly in the pathways of DNA damage signaling and homology-directed DNA double strand break repair. This study highlights the importance of utilizing rhesus monkey to explore the molecular basis of genome reprogramming, ZGA, and genomic stability surveillance in human early embryogenesis, and provides important clues on how to improve the efficiency of homologous recombination-mediated gene editing in monkey transgenic technology. |
| 资助信息 | This work was supported by the "Strategic Priority Research Program" of the CAS ， Grant No. XDA01010203 and XDB13000000 to P.Z, and grants from CAS XDA01010303, China Ministry of Science and Technology 2015CB964803 and National Natural Science Foundation of China 91329302, 31210103916 and 91519330 to J.D.J.H. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/10893]  |
| 专题 | 昆明动物研究所_哺乳动物胚胎发育 昆明动物研究所_遗传资源与进化国家重点实验室 昆明动物研究所_中国科学院昆明灵长类研究中心 |
| 作者单位 | 1.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China 2.Key Laboratory of Computational Biology, CAS Center for Excellence in Molecular Cell Science, Collaborative Innovation Center for Genetics and Developmental Biology, Chinese Academy of Sciences-Max Planck Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China 3.Yunnan Key Laboratory of Animal Reproduction, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China 4.University of Chinese Academy of Sciences, Beijing, China 5.Kunming College of Life Science, University of Chinese Academy of Sciences, Kunming, Yunnan 650204, China 6.Primate Research Center, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650223, China |
| 推荐引用方式 GB/T 7714 | Wang XY,Liu DH,He XC,et al. Transcriptome analyses of rhesus monkey pre-implantation embryos reveal a reduced capacity for DNA double strand break repair in primate oocytes and early embryos[J]. GENOME RESEARCH,2017,27(X):567–579. |
| APA | Wang XY.,Liu DH.,He XC.,Han JD[*].,Zheng P[*].,...&Xia X.(2017).Transcriptome analyses of rhesus monkey pre-implantation embryos reveal a reduced capacity for DNA double strand break repair in primate oocytes and early embryos.GENOME RESEARCH,27(X),567–579. |
| MLA | Wang XY,et al."Transcriptome analyses of rhesus monkey pre-implantation embryos reveal a reduced capacity for DNA double strand break repair in primate oocytes and early embryos".GENOME RESEARCH 27.X(2017):567–579. |
入库方式： OAI收割
来源：昆明动物研究所
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