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Peptidomics combined with cDNA library unravel the diversity of centipede venom

文献类型：期刊论文

作者Rong MQ1; Yang SL1; Wen B3; Mo GX2; Kang D1; Liu J1; Lin ZL3; Jiang WB4; Li BW1; Du CQ3
刊名JOURNAL OF PROTEOMICS
出版日期2015
卷号114期号:X页码:28-37
关键词Centipede Toxin Diversity Peptidomics
通讯作者wangj@genomics.cn ; rlai@mail.kiz.ac.cn
合作状况其它
英文摘要Centipedes are one of the oldest venomous arthropods using toxin as their weapon to capture prey. But little attention was focused on them and only few centipede toxins were demonstrated with activity on ion channels. Therefore, more deep works are needed to understand the diversity of centipede venom. In the present study, we use peptidomics combined with cDNA library to uncover the diversity of centipede Scolopendra subspinipes mutilans L. Koch. 192 peptides were identified by LC-MS/MS and 79 precursors were deduced by cDNA library. Surprisingly, the signal peptides of centipede toxins were more complicated than any other animal toxins and even exhibited large differences in homologues. Meanwhile, a large number of variants generated by alternative cleavage sites were detected by mass spectra. Odd number of cystein (3, 5, 7) found in the mature peptides were seldom seen in peptide toxins. In additional, two novel cysteine frameworks (C-C-C-CCC, C-C-C-C-CC-CC) were identified from 16 different cysteine frameworks from centipede peptides. Only 29 precursors have clear targets, while others may provide a potential diversity function for centipede. These findings highlight the extensive diversity of centipede toxins and provide powerful tools to understand the capture and defense weapon of centipede. Biological significance Peptide toxins from venomous animal have attracted increasing attentions due to their extraordinary chemical and pharmacological diversity. Centipedes are one of the most used Chinese traditional medicines, but little was known about the active components. The venom of Scolopendra subspinipes mutilans L. Koch is first deeply analyzed in this work and most of peptides were never discovered before. Interestingly, the number and arrangement of cysteine showed a larger different to known peptide toxins such spider or scorpion toxins. Moreover, only 29 peptides from this centipede venom were identified with known function. It suggested that our work not only important to understand the composition of centipede venom, but also provide many valuable peptides for potential biological functions
收录类别SCI
资助信息This work was supported by the Chinese National Natural Science Foundation (30830021, 31025025, 31070701, 31000960, 31025025, U1132601, 31200590), and the Ministry of Science and Technology (2010CB529800, 2011ZX09102-002-10).
语种英语
WOS记录号WOS:000348949100003
公开日期2015-03-06
源URL[http://159.226.149.42:8088/handle/152453/8273]  
专题昆明动物研究所_动物毒素室
昆明动物研究所_动物模型与人类重大疾病机理重点实验室
管理、支撑部门_大型仪器中心
作者单位1.Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming 650223, Yunnan, China
2.School of Biological Sciences, Nanjing Agriculture University, Nanjing, Jiangshu 210095, China
3.BGI-Shenzhen, Shenzhen 518083, China
4.College of Life Science and Technology, Kunming University of Science and Technology, China
5.Kunming Biological Diversity Regional Center of Large Apparatuses and Equipment, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China
6.Princess Al Jawhara Center of Excellence in the Research of Hereditary Disorders, King Abdulaziz University, Jeddah, Saudi Arabia
7.The Novo Nordisk Foundation Center for Basic Metabolic Research, University of Copenhagen, Copenhagen, Denmark
推荐引用方式
GB/T 7714		 Rong MQ,Yang SL,Wen B,et al. Peptidomics combined with cDNA library unravel the diversity of centipede venom[J]. JOURNAL OF PROTEOMICS,2015,114(X):28-37.
APA Rong MQ.,Yang SL.,Wen B.,Mo GX.,Kang D.,...&Lai R[*].(2015).Peptidomics combined with cDNA library unravel the diversity of centipede venom.JOURNAL OF PROTEOMICS,114(X),28-37.
MLA Rong MQ,et al."Peptidomics combined with cDNA library unravel the diversity of centipede venom".JOURNAL OF PROTEOMICS 114.X(2015):28-37.

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来源：昆明动物研究所

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