中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
鱇(鱼良)白鱼线粒体DNA控制区结构和种群遗传多样性分析

文献类型:期刊论文

作者杨博1,2; 陈小勇[*]1; 杨君兴[*]1
刊名动物学研究
出版日期2008
卷号29期号:4页码:379-385
关键词鱇(鱼良)白鱼 线粒体DNA 控制区 传多样性
其他题名Structure of the Mitochondrial DNA Control Region and Population Genetic Diversity Analysis of Anabarilius grahami(Regan)
通讯作者chenxy@mail.kiz.ac.cn ; yangjx@mail.kiz.ac.cn
合作状况其它
中文摘要用特异性引物对鱇(鱼良)白鱼(Anabarilius grahami)DNA进行PCR扩增,获得了鱇(鱼良)白鱼线粒体DNA控制区基因全序列(930bp)。控制区T、C、A和G碱基组成为29.8%、22.5%、33.0%和14.7%。对照其他已报道的鱼类控制区结构,对鱇(鱼良)白鱼控制区结构进行了分析,识别了其终止序列区、中央保守区和保守序列区,找到了终止相关的序列TAS以及保守序列(CSB-F、CSB-D、CSB-1、CSB-2、CSB-3)。同时运用DNA分析软件对白鱼一个驯养种群(中国科学院昆明动物研究所珍稀鱼类繁育中心)及两个自然地理种群(江川县明星鱼洞、江川县牛摩村)进行了遗传多样性分析。结果显示:两个自然种群存在较强基因交流,未出现遗传分化;人工驯养种群遗传多样性最高,种群复壮程度较好。
英文摘要The control region (D-loop) of Anabarilius grahami was amplified by PCR amplification with a pair of specific primers. The sequence with the complete nucleotide control region from A. grahami mitochondrial was cloned and directly sequenced. The length of this region (D-loop) contained 930bp nucleotides and the T, C, A and G contents were 29.8%, 22.5%, 33.0% and 14.7% respectively. The mtDNA control region ofA. grahami could be partitioned into three domains, namely, the termination associated sequence domain, the central conserved sequence domain and the conserved sequence block domain. The extended termination associated sequence (ETAS), two conserved blocks (CSB-F, CSB-D) in the central conserved sequence block domain and three conserved sequence blocks (CSBI, CSB2, CSB3) in the conserved sequence block domain were successfully identified and their homology compared with other fish. The genetic diversity analysis ofA. grahami from the Endangered Fish Breeding Center (EFBC) of the Kunming Institute of Zoology (KIZ), Mingxing Fish Cave (Jiangchuan), Niumo Village (Jiangchuan) was analyzed. The result indicated a very low genetic divergence between two natural populations, and the genetic diversity of the population from EFBC was much higher; they had recovered better than others.
收录类别其他
资助信息国家自然科学基金霞点项目(30730017):国家重点基础研究发展计划(2007CB411600)
语种中文
公开日期2010-08-04
源URL[http://159.226.149.42:8088/handle/152453/893]  
专题昆明动物研究所_系统进化与生物地理学
昆明动物研究所_东南亚野生动物多样性
作者单位1.中国科学院昆明动物研究所,云南昆明650223
2.中国科学院研究生院,北京100049
推荐引用方式
GB/T 7714
杨博,陈小勇[*],杨君兴[*]. 鱇(鱼良)白鱼线粒体DNA控制区结构和种群遗传多样性分析[J]. 动物学研究,2008,29(4):379-385.
APA 杨博,陈小勇[*],&杨君兴[*].(2008).鱇(鱼良)白鱼线粒体DNA控制区结构和种群遗传多样性分析.动物学研究,29(4),379-385.
MLA 杨博,et al."鱇(鱼良)白鱼线粒体DNA控制区结构和种群遗传多样性分析".动物学研究 29.4(2008):379-385.

入库方式: OAI收割

来源:昆明动物研究所

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