4 种渗透性抗冻剂对暗色唇鱼精子冷冻保存的影响
文献类型:期刊论文
作者 | 王晓爱1,2; 杨君兴[*]1; 陈小勇[*]1; 潘晓赋1; 李再云1 |
刊名 | 水生态学杂志
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出版日期 | 2012 |
卷号 | 33期号:5页码:88-93 |
关键词 | 渗透性抗冻剂 暗色唇鱼 精子冷冻 |
其他题名 | Effects of Four Penetrating Cryoprotectants on Cryopreservation of Semilabeo obscurus Sperm |
通讯作者 | chenxy@mail.kiz.ac.cn ; yangjx@mail.kiz.ac.cn |
合作状况 | 其它 |
中文摘要 | 4℃冷冻保存条件下,以冷冻精子活力、寿命和细胞膜完整率为指标,检测二甲亚砜(DMSO)、甘油(Glycerol)、乙二醇(EG)和甲醇(MeOH)4种渗透性抗冻剂对暗色唇鱼(Semilabeo obscurus)鲜精以及对精子冷冻保存效果的影响。结果显示,4种渗透性抗冻剂对鲜精活力呈负作用,MeOH |
英文摘要 | The present study aimed to examine effects of four penetrating cryoprotectants (dimethyl sulfoxide,glyc- erol,ethylene glycol and methanol) on fresh and frozen-thawed (freezing temperature was 4℃) sperms of Semila- beo obscurus. The function of fresh and frozen-thawed sperms was evaluated by sperm motility,sperm life and sperm membrane integrity. Results obtained here showed that: four penetrating cryoprotectants presented different negative effects on fresh sperm motility and membrane integrity,methanol < ethylene glycol < dimethyl sulfoxide < glycerol; negative effects on fresh sperm life were also observed,methanol < ethylene glycol < glycerol and dimethyl sulfox- ide; fresh sperms of S. obscurus [with (36. 17 ± 4. 06)% for motility,(47. 5 ± 3. 1)s for life,and (86. 22 ± 5. 29)% for membrane integrity]were frozen for a short - term; the post-thawing motility of frozen sperms treated with 5% methanol or 5% ethylene glycol were respectively (18. 33 ±1. 70)% and (17. 83 ±2. 67)%,which were significant different with the fresh sperm motility and higher than those frozen sperms with other treatments; the post-thawing life of frozen sperms treated with 5% methanol or 5% ethylene glycol were respectively (44. 3 ± 3. 6) s and (42. 8 ±5. 5) s,which were longer than those frozen sperms with other treatments; there was no signif- icant difference between post-thawing life of frozen sperms treated with 5% methanol and fresh sperm life; In terms of sperm membrane integrity,5% methanol was the optimal cryoprotectants,which had no significant differences with fresh sperm membrane integrity. These results indicated that: 1) dimethyl sulfoxide and glycerol were not the suitable cryoprotectants for S. obscurus sperm cryopreservation; 2) methanol and ethylene glycol had similar effects,which were potential penetrating cryoprotectants for S. obscurus sperm cryopreservation. |
收录类别 | 其他 |
资助信息 | 云南大唐国际李仙江流域水电开发有限公司委托项目. |
语种 | 中文 |
公开日期 | 2013-06-18 |
源URL | [http://159.226.149.42:8088/handle/152453/7489] ![]() |
专题 | 昆明动物研究所_系统进化与生物地理学 昆明动物研究所_遗传资源与进化国家重点实验室 昆明动物研究所_东南亚野生动物多样性 |
作者单位 | 1.中国科学院昆明动物研究所 遗传资源与进化国家重点实验室,云南昆明650223 2.中国科学院研究生院;北京100049 |
推荐引用方式 GB/T 7714 | 王晓爱,杨君兴[*],陈小勇[*],等. 4 种渗透性抗冻剂对暗色唇鱼精子冷冻保存的影响[J]. 水生态学杂志,2012,33(5):88-93. |
APA | 王晓爱,杨君兴[*],陈小勇[*],潘晓赋,&李再云.(2012).4 种渗透性抗冻剂对暗色唇鱼精子冷冻保存的影响.水生态学杂志,33(5),88-93. |
MLA | 王晓爱,et al."4 种渗透性抗冻剂对暗色唇鱼精子冷冻保存的影响".水生态学杂志 33.5(2012):88-93. |
入库方式: OAI收割
来源:昆明动物研究所
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