中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
4 种渗透性抗冻剂对暗色唇鱼精子冷冻保存的影响

文献类型:期刊论文

作者王晓爱1,2; 杨君兴[*]1; 陈小勇[*]1; 潘晓赋1; 李再云1
刊名水生态学杂志
出版日期2012
卷号33期号:5页码:88-93
关键词渗透性抗冻剂 暗色唇鱼 精子冷冻
其他题名Effects of Four Penetrating Cryoprotectants on Cryopreservation of Semilabeo obscurus Sperm
通讯作者chenxy@mail.kiz.ac.cn ; yangjx@mail.kiz.ac.cn
合作状况其它
中文摘要4℃冷冻保存条件下,以冷冻精子活力、寿命和细胞膜完整率为指标,检测二甲亚砜(DMSO)、甘油(Glycerol)、乙二醇(EG)和甲醇(MeOH)4种渗透性抗冻剂对暗色唇鱼(Semilabeo obscurus)鲜精以及对精子冷冻保存效果的影响。结果显示,4种渗透性抗冻剂对鲜精活力呈负作用,MeOH0.05);5%MeOH解冻后精子细胞膜完整率最高,为(85.67±1.11)%,与鲜精细胞膜完整率无显著差异(P>0.05)。研究表明,DMSO和Glycerol并不适合作为暗色唇鱼精子冷冻的抗冻剂,MeOH和EG具有相似的保护作用,是暗色唇鱼精子冷冻保存潜在的渗透性抗冻剂。
英文摘要The present study aimed to examine effects of four penetrating cryoprotectants (dimethyl sulfoxide,glyc- erol,ethylene glycol and methanol) on fresh and frozen-thawed (freezing temperature was 4℃) sperms of Semila- beo obscurus. The function of fresh and frozen-thawed sperms was evaluated by sperm motility,sperm life and sperm membrane integrity. Results obtained here showed that: four penetrating cryoprotectants presented different negative effects on fresh sperm motility and membrane integrity,methanol < ethylene glycol < dimethyl sulfoxide < glycerol; negative effects on fresh sperm life were also observed,methanol < ethylene glycol < glycerol and dimethyl sulfox- ide; fresh sperms of S. obscurus [with (36. 17 ± 4. 06)% for motility,(47. 5 ± 3. 1)s for life,and (86. 22 ± 5. 29)% for membrane integrity]were frozen for a short - term; the post-thawing motility of frozen sperms treated with 5% methanol or 5% ethylene glycol were respectively (18. 33 ±1. 70)% and (17. 83 ±2. 67)%,which were significant different with the fresh sperm motility and higher than those frozen sperms with other treatments; the post-thawing life of frozen sperms treated with 5% methanol or 5% ethylene glycol were respectively (44. 3 ± 3. 6) s and (42. 8 ±5. 5) s,which were longer than those frozen sperms with other treatments; there was no signif- icant difference between post-thawing life of frozen sperms treated with 5% methanol and fresh sperm life; In terms of sperm membrane integrity,5% methanol was the optimal cryoprotectants,which had no significant differences with fresh sperm membrane integrity. These results indicated that: 1) dimethyl sulfoxide and glycerol were not the suitable cryoprotectants for S. obscurus sperm cryopreservation; 2) methanol and ethylene glycol had similar effects,which were potential penetrating cryoprotectants for S. obscurus sperm cryopreservation.
收录类别其他
资助信息云南大唐国际李仙江流域水电开发有限公司委托项目.
语种中文
公开日期2013-06-18
源URL[http://159.226.149.42:8088/handle/152453/7489]  
专题昆明动物研究所_系统进化与生物地理学
昆明动物研究所_遗传资源与进化国家重点实验室
昆明动物研究所_东南亚野生动物多样性
作者单位1.中国科学院昆明动物研究所 遗传资源与进化国家重点实验室,云南昆明650223
2.中国科学院研究生院;北京100049
推荐引用方式
GB/T 7714
王晓爱,杨君兴[*],陈小勇[*],等. 4 种渗透性抗冻剂对暗色唇鱼精子冷冻保存的影响[J]. 水生态学杂志,2012,33(5):88-93.
APA 王晓爱,杨君兴[*],陈小勇[*],潘晓赋,&李再云.(2012).4 种渗透性抗冻剂对暗色唇鱼精子冷冻保存的影响.水生态学杂志,33(5),88-93.
MLA 王晓爱,et al."4 种渗透性抗冻剂对暗色唇鱼精子冷冻保存的影响".水生态学杂志 33.5(2012):88-93.

入库方式: OAI收割

来源:昆明动物研究所

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