4 种渗透性抗冻剂对暗色唇鱼精子冷冻保存的影响
文献类型：期刊论文
| 作者 | 王晓爱1,2; 杨君兴[*]1; 陈小勇[*]1; 潘晓赋1; 李再云1 |
| 刊名 | 水生态学杂志
 |
| 出版日期 | 2012 |
| 卷号 | 33期号:5页码:88-93 |
| 关键词 | 渗透性抗冻剂 暗色唇鱼 精子冷冻 |
| 其他题名 | Effects of Four Penetrating Cryoprotectants on Cryopreservation of Semilabeo obscurus Sperm |
| 通讯作者 | chenxy@mail.kiz.ac.cn ; yangjx@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 中文摘要 | 4℃冷冻保存条件下,以冷冻精子活力、寿命和细胞膜完整率为指标,检测二甲亚砜(DMSO)、甘油(Glycerol)、乙二醇(EG)和甲醇(MeOH)4种渗透性抗冻剂对暗色唇鱼(Semilabeo obscurus)鲜精以及对精子冷冻保存效果的影响。结果显示,4种渗透性抗冻剂对鲜精活力呈负作用,MeOH |
| 英文摘要 | The present study aimed to examine effects of four penetrating cryoprotectants (dimethyl sulfoxide，glyc- erol，ethylene glycol and methanol) on fresh and frozen-thawed (freezing temperature was 4℃) sperms of Semila- beo obscurus． The function of fresh and frozen-thawed sperms was evaluated by sperm motility，sperm life and sperm membrane integrity． Results obtained here showed that: four penetrating cryoprotectants presented different negative effects on fresh sperm motility and membrane integrity，methanol ＜ ethylene glycol ＜ dimethyl sulfoxide ＜ glycerol; negative effects on fresh sperm life were also observed，methanol ＜ ethylene glycol ＜ glycerol and dimethyl sulfox- ide; fresh sperms of S． obscurus ［with (36． 17 ± 4． 06)% for motility，(47． 5 ± 3． 1)s for life，and (86． 22 ± 5． 29)% for membrane integrity］were frozen for a short － term; the post-thawing motility of frozen sperms treated with 5% methanol or 5% ethylene glycol were respectively (18． 33 ±1． 70)% and (17． 83 ±2． 67)%，which were significant different with the fresh sperm motility and higher than those frozen sperms with other treatments; the post-thawing life of frozen sperms treated with 5% methanol or 5% ethylene glycol were respectively (44． 3 ± 3． 6) s and (42． 8 ±5． 5) s，which were longer than those frozen sperms with other treatments; there was no signif- icant difference between post-thawing life of frozen sperms treated with 5% methanol and fresh sperm life; In terms of sperm membrane integrity，5% methanol was the optimal cryoprotectants，which had no significant differences with fresh sperm membrane integrity． These results indicated that: 1) dimethyl sulfoxide and glycerol were not the suitable cryoprotectants for S． obscurus sperm cryopreservation; 2) methanol and ethylene glycol had similar effects，which were potential penetrating cryoprotectants for S． obscurus sperm cryopreservation． |
| 收录类别 | 其他 |
| 资助信息 | 云南大唐国际李仙江流域水电开发有限公司委托项目. |
| 语种 | 中文 |
| 公开日期 | 2013-06-18 |
| 源URL | [http://159.226.149.42:8088/handle/152453/7489]  |
| 专题 | 昆明动物研究所_系统进化与生物地理学 昆明动物研究所_遗传资源与进化国家重点实验室 昆明动物研究所_东南亚野生动物多样性 |
| 作者单位 | 1.中国科学院昆明动物研究所 遗传资源与进化国家重点实验室，云南昆明650223 2.中国科学院研究生院;北京100049 |
| 推荐引用方式 GB/T 7714 | 王晓爱,杨君兴[*],陈小勇[*],等. 4 种渗透性抗冻剂对暗色唇鱼精子冷冻保存的影响[J]. 水生态学杂志,2012,33(5):88-93. |
| APA | 王晓爱,杨君兴[*],陈小勇[*],潘晓赋,&李再云.(2012).4 种渗透性抗冻剂对暗色唇鱼精子冷冻保存的影响.水生态学杂志,33(5),88-93. |
| MLA | 王晓爱,et al."4 种渗透性抗冻剂对暗色唇鱼精子冷冻保存的影响".水生态学杂志 33.5(2012):88-93. |
入库方式： OAI收割
来源：昆明动物研究所
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