大蹼铃蟾皮肤分泌液中抗菌活性肽的分离纯化及其性质
文献类型：期刊论文
| 作者 | 赖仞; 叶文娟; 冉永禄[*]; 吕顺清; 杨大同 |
| 刊名 | 动物学研究
 |
| 出版日期 | 1998 |
| 卷号 | 19期号:4页码:257-262 |
| 关键词 | 大蹼铃蟾 抗菌皮肤肽 分离纯化 性质 |
| ISSN号 | 0254-5853 |
| 其他题名 | Anti-Microbial Peptide From The Skin Secretion of Bombina maxima |
| 中文摘要 | 从云南产大蹼铃蟾( Bombina maxima)皮肤分泌液中分离得到了一种有广泛抗菌活性的多肽,被命名为MSP-VII。用快原子轰击质谱(f.a.b.m.s)分析其M+/Z=2648,即此活性肽的分子量为2647D。用自动氨基酸分析仪分析,含有26个氨基酸。抗菌实验结果表明,此多肽对格兰氏阴性和格兰氏阳性细菌以及真菌都有较强的抗菌活性。溶血实验表明此活性肽有很轻微的溶血活性。此外,MSP-VII也可以促进平滑肌收缩和增加微血管的渗透性。 |
| 英文摘要 | An anti-microbial peptide named MSP-Ⅶ,was isolated from the skin secretion of the toad,Bombina maxima,distributed in Yunnan Province of China,by a three step protocol including Sephadex G-75 and G-50 connected with G-25 two steps of the gel filtration and one reverse-phase high-performance liquid chromatography.Fast atom bombardment mass-spectroscopy of MSP-Ⅶ gave a protonated molecular ion m/z 2648 which matched the theoretical molecular weight predicted from the composition of amino acids.It consists of twenty-six amino acid residues determined by automatic amino acid analyzer.MSP-Ⅶ is a water-soluble peptide.MSP-Ⅶ has a strong anti-microbial activity to bacteria,fungi and yeast with a little hemolysis activity.These characterizations of MSP-Ⅶ make it possible to be used as a clinical anti-microbial medicine.We tested its effect on micro-vessel osmosis ability with Evens blue dye.It was found that MSP-Ⅶ made the permeability of some tissues such as lung,liver,small intestine,to Evens blue dye increase.In particular,this peptide obviously increased permeability of lung.In addition,MSP-Ⅶ displayed an effect on the contraction of smooth muscle,which strengthened the contraction ability of rabbit aorta smooth muscle. |
| 收录类别 | 其他 |
| 资助信息 | 云南省应用基础基金资助,热区联合开发中心资助 |
| 原文出处 | 1998194257.pdf |
| 语种 | 中文 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/1971]  |
| 专题 | 昆明动物研究所_动物毒素室 昆明动物研究所_其他 |
| 作者单位 | 中国科学院昆明动物研究所,昆明650223 |
| 推荐引用方式 GB/T 7714 | 赖仞,叶文娟,冉永禄[*],等. 大蹼铃蟾皮肤分泌液中抗菌活性肽的分离纯化及其性质[J]. 动物学研究,1998,19(4):257-262. |
| APA | 赖仞,叶文娟,冉永禄[*],吕顺清,&杨大同.(1998).大蹼铃蟾皮肤分泌液中抗菌活性肽的分离纯化及其性质.动物学研究,19(4),257-262. |
| MLA | 赖仞,et al."大蹼铃蟾皮肤分泌液中抗菌活性肽的分离纯化及其性质".动物学研究 19.4(1998):257-262. |
入库方式： OAI收割
来源：昆明动物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。