Identification and cloning of a trypsin inhibitor from skin secretions of Chinese red-belly toad Bombina maxima
文献类型：期刊论文
| 作者 | Lai R; Liu H; Lee WH; Zhang Y[*] |
| 刊名 | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY Part B
 |
| 出版日期 | 2002 |
| 卷号 | 131期号:1页码:47-53 |
| 关键词 | Protease inhibitor Trypsin Amphibian skin Bombina Toad Bombina maxima trypsin inhibitor BMTI cDNA Sequence |
| ISSN号 | 1096-4959 |
| 通讯作者 | zhangy@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | A novel trypsin inhibitor was identified and purified from skin secretions of Chinese red-belly toad Bombina maxima. The partial N-terminal 29 amino acid residues of the peptide, named BMTI, were determined by automated Edman degradation. This allowed the cloning of a full-length cDNA encoding BMTI from a cDNA library prepared from the toad skin. The deduced complete amino acid sequence of BMTI indicates that mature BMTI is composed of 60 amino acids. A FASTA search in the databanks revealed that BMTI exhibits 81.7% sequence identity with BSTI, a trypsin/thrombin inhibitor from European toad Bombina bombina skin secretions. Sequence differences between BMTI and BSTI were due to 11 substitutions at positions 2, 9, 25, 27, 36-37, 39, 41-42, 50 and 56. BMTI potently inhibited trypsin with a K(i) value of 0.06 microM, similar to that of BSTI. However, unlike BSTI, which also inhibited thrombin with a K(i) value of 1 microM, no inhibitory effect of BMTI on thrombin was observed under the assay conditions. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by grants from ‘STZ98-3-01’, ‘Western Light Project’ and ‘Tenth Five Plan’ pre-research projects from the Chinese Academy of Sciences and a grant from National Ž . Natural Science foundation 30170195 . |
| 原文出处 | 200213147.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/2471]  |
| 专题 | 昆明动物研究所_动物毒素室 昆明动物研究所_动物活性蛋白多肽组学 |
| 作者单位 | Department of Animal Toxinology, Kunming Institute of Zoology, Chinese Academy of Sciences, 32 East Jiao Chang Road, Kunming 650223, Yunnan, PR China |
| 推荐引用方式 GB/T 7714 | Lai R,Liu H,Lee WH,et al. Identification and cloning of a trypsin inhibitor from skin secretions of Chinese red-belly toad Bombina maxima[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY Part B,2002,131(1):47-53. |
| APA | Lai R,Liu H,Lee WH,&Zhang Y[*].(2002).Identification and cloning of a trypsin inhibitor from skin secretions of Chinese red-belly toad Bombina maxima.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY Part B,131(1),47-53. |
| MLA | Lai R,et al."Identification and cloning of a trypsin inhibitor from skin secretions of Chinese red-belly toad Bombina maxima".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY Part B 131.1(2002):47-53. |
入库方式： OAI收割
来源：昆明动物研究所
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