Anti-infection peptidomics of amphibian skin
文献类型：期刊论文
| 作者 | Li JX1,2,3; Xu XQ1,2,3; Xu CH4; Zhou WP2,5; Zhang KY4; Yu HN6; Zhang YP5; Zheng YT7; Rees HH8; Lai R[*]1,4,9 |
| 刊名 | MOLECULAR & CELLULAR PROTEOMICS
 |
| 出版日期 | 2007 |
| 卷号 | 6期号:5页码:882-894 |
| ISSN号 | 1535-9476 |
| 通讯作者 | rlai@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | Peptidomics and genomics analyses were used to study an anti-infection array of peptides of amphibian skin. 372 cDNA sequences of antimicrobial peptides were characterized from a single individual skin of the frog Odorrana grahami that encode 107 novel antimicrobial peptides. This contribution almost triples the number of currently reported amphibian antimicrobial peptides. The peptides could be organized into 30 divergent groups, including 24 novel groups. The diversity in peptide coding cDNA sequences is, to our knowledge, the most extreme yet described for any animal. The patterns of diversification suggest that point mutations as well as insertion, deletion, and "shuffling" of oligonucleotide sequences were responsible for the diversity. The diversity of antimicrobial peptides may have resulted from the diversity of microorganisms. These diverse peptides exhibited both diverse secondary structure and "host defense" properties. Such extreme antimicrobial peptide diversity in a single amphibian species is amazing. This has led us to reconsider the strong capability of innate immunity and molecular genetics of amphibian ecological diversification and doubt the general opinion that 20-30 different antimicrobial peptides can protect an animal because of the relatively wide specificity of the peptide antibiotics. The antimicrobial mechanisms of O. grahami peptides were investigated. They exerted their antimicrobial functions by various means, including forming lamellar mesosome-like structures, peeling off the cell walls, forming pores, and inducing DNA condensation. With respect to the development of antibiotics, these peptides provide potential new templates to explore further. |
| 学科主题 | Biochemical Research Methods |
| 收录类别 | SCI |
| 资助信息 | This work was supported by Chinese National Natural Science Foundation Grants 30570360 and 30670456, Yunnan Natural Science Foundation Grants 2005C0054M and 2006C0011Z, Chinese Acad- emy of Sciences Grant KSCX2-YW-R-20, and Jiangsu Natural Sci- ence Foundation Grant BK2005422. |
| 原文出处 | 20076882.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/2513]  |
| 专题 | 昆明动物研究所_动物毒素室 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 昆明动物研究所_分子进化基因组学 昆明动物研究所_分子免疫药理学 昆明动物研究所_细胞与分子进化重点实验室 |
| 作者单位 | 1.Biotoxin Units,Key Laboratory of Animal Models and Human Disease Mechanisms,Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China 2.Graduate School of the Chinese Academy of Sciences, Beijing 100009, China 3.Chinese Acad Sci, Kunming Inst Zool, Kunming 650223, Yunnan, Peoples R China 4.Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing 210095, Jiangsu, China 5.Key Laboratory of Cellular and Molecular Evolu- tion, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China 6.College of Life Sciences of Hebei Normal University, Shijiazhuang 050016, Hebei, China 7.Laboratory of Molecular Immunop- harmacology, Key Laboratory of Animal Models and Human Disease Mechanisms,Kunming Institute of Zoology, Chinese Academy of Sciences 8.School of Biological Sciences University of Liver- pool, Liverpool L69 7ZB, United Kingdom 9.Beijing Inst Biomed, Beijing 100091, Peoples R Chin 10.Beijing Institute of Biomedicine, 15 Xinjiangongmen Rd., Beijing 100091, China |
| 推荐引用方式 GB/T 7714 | Li JX,Xu XQ,Xu CH,et al. Anti-infection peptidomics of amphibian skin[J]. MOLECULAR & CELLULAR PROTEOMICS,2007,6(5):882-894. |
| APA | Li JX.,Xu XQ.,Xu CH.,Zhou WP.,Zhang KY.,...&Wu J.(2007).Anti-infection peptidomics of amphibian skin.MOLECULAR & CELLULAR PROTEOMICS,6(5),882-894. |
| MLA | Li JX,et al."Anti-infection peptidomics of amphibian skin".MOLECULAR & CELLULAR PROTEOMICS 6.5(2007):882-894. |
入库方式： OAI收割
来源：昆明动物研究所
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