Molecular Phylogeny of Geographical Isolates of Bursaphelenchus xylophilus: Implications on the Origin and Spread of this Species in China and Worldwide
文献类型：期刊论文
| 作者 | Zhang KY[*]1,2; Liu H1,2; Sun J1; Liu JR1,2; Fei K1,2; Zhang CX1; Xu MX1; Sun J1; Ma XY1; Lai R1,3 |
| 刊名 | JOURNAL OF NEMATOLOGY
 |
| 出版日期 | 2008 |
| 卷号 | 40期号:2页码:127-137 |
| 关键词 | Bursaphelenchus xylophilus ITS phylogeny pinewood nematode RAPD systematics D2/3 regions of 28S rDNA |
| ISSN号 | 0022-300X |
| 通讯作者 | keyunzhang@njau.edu.cn |
| 合作状况 | 其它 |
| 英文摘要 | The genetic diversity and phylogeny of 26 isolates of Bursaphelenchus xlophilus from China, Japan, Portugal and North America were investigated based on the D2/3 domain of 28S rDNA, nuclear ribosomal Internal Transcribed Spacer (ITS) sequences, and random amplified polymorphic DNA (RAPD) analysis. The genetic diversity analysis showed that the D2/3 domain of 28S rDNA of isolates of B. xlophilus from China, Portugal, Japan and the US were identical and differed at one to three nucleotides compared to those from Canada. ITS sequences of isolates from China and Portugal were the same; they differed at one or two nucleotides compared to those of Japanese isolates and at four and 23 nucleotides compared to those front the US and Canada, respectively. The phylogenetic analysis indicated that Chinese isolates share a common ancestor with one of the two Japanese clades and that the Canadian isolates form a sister group of the clade comprised of isolates from China, Portugal,Japan, and the US. The relationship between Japanese isolates and those from China was closer than with the American isolates. The Canadian isolates were the basal group of B. xylophilus. This suggests that B. xlophilus originated in North America and that the B. xylphilus that occurs in China could have been first introduced from Japan. Further analysis based on RAPD analysis revealed that the relationship among isolates from Guangdong, Zhejiang, Shandong, Anhui provinces and Nanjing was the closest, which suggests that pine wilt disease in these Chinese locales was probably dispersed from Nanjing, where this disease first occurred in China. |
| 收录类别 | SCI |
| 资助信息 | This research was supported by the China Post-doctoral Science Foundation (20060390292), Jiangsu Province Natural Science Foundation (BK2006560) and Jiangsu Province Post-doctoral Science Foundation (0601030B). |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/2521]  |
| 专题 | 昆明动物研究所_动物毒素室 |
| 作者单位 | 1.Department of Zoology, College of Life Sciences, Nanjing Agricultural Uni- versity, Nanjing, 210095, China 2.Key laboratory of Monitoring and Management of Plant Disease and In- sects, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, 210095, China 3.Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650223, China |
| 推荐引用方式 GB/T 7714 | Zhang KY[*],Liu H,Sun J,et al. Molecular Phylogeny of Geographical Isolates of Bursaphelenchus xylophilus: Implications on the Origin and Spread of this Species in China and Worldwide[J]. JOURNAL OF NEMATOLOGY,2008,40(2):127-137. |
| APA | Zhang KY[*].,Liu H.,Sun J.,Liu JR.,Fei K.,...&Lin MS.(2008).Molecular Phylogeny of Geographical Isolates of Bursaphelenchus xylophilus: Implications on the Origin and Spread of this Species in China and Worldwide.JOURNAL OF NEMATOLOGY,40(2),127-137. |
| MLA | Zhang KY[*],et al."Molecular Phylogeny of Geographical Isolates of Bursaphelenchus xylophilus: Implications on the Origin and Spread of this Species in China and Worldwide".JOURNAL OF NEMATOLOGY 40.2(2008):127-137. |
入库方式： OAI收割
来源：昆明动物研究所
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