A novel bradykinin-like peptide from skin secretions of the frog, Rana nigrovittata
文献类型：期刊论文
| 作者 | Liu XH1; You DW2,3; Chen LH1; Wang X1; Zhang KY1; Lai R[*]1,2 |
| 刊名 | JOURNAL OF PEPTIDE SCIENCE
 |
| 出版日期 | 2008 |
| 卷号 | 14期号:5页码:626-630 |
| 关键词 | amphibian ranakinin-N bradykinin-like peptide Rana nigrovittata skin |
| ISSN号 | 1075-2617 |
| 通讯作者 | rlai72@njau.edu.cn |
| 合作状况 | 其它 |
| 英文摘要 | A bradykinin-like peptide has been isolated from the skin secretions of the frog Rana nigrovittata. This peptide was named ranakinin-N. Its primary structure, RAEAVPPGFTPFR, was determined by Edman degradation and mass spectrometry. It is structurally related to bradykinin-like peptides identified from skin secretions of other amphibians. Ranakinin-N is composed of 13 amino acid residues and is related to the bradykinin identified from the skin secretions of Odorrana schmackeri, which is composed of 9 amino acid residues. Ranakinin-N was found to exert concentration-dependent contractile effects on isolated guinea pig ileum. cDNA sequence encoding the precursor of ranakinin-N was isolated from a skin cDNA library of R. nigrovittata. The amino acid sequences deduced from the cDNA sequences match well with the results from Edman degradation. Analysis of different amphibian bradykinin cDNA structures revealed that the deficiency of a 15-nucleotide fragment (agaatgatcagacgc in the cDNA encoding bradykinin from O. schmackeri) in the peptide-coding region resulted in the absence of a dibasic site for trypsin-like proteinases and an unusual -AEVA- insertion in the N-terminal part of ranakinin-N. The -AEAV- insertion resulted in neutral net charge at the N-terminus of ranakinin-N. Ranakinin-N is the first reported bradykinin-like peptide with a neutral net charge at the N-terminus. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by Jiangsu Natural Science Foundation (BK2005422), the Ministry of Science and Technology of the People’s Republic of China (2006BAD06B07) and the Chinese Academy of Sciences (KSCX2-YW-R-20 and KSCX2-YW-G-024). |
| 原文出处 | 200814626.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/2537]  |
| 专题 | 昆明动物研究所_动物毒素室 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 |
| 作者单位 | 1.Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China 2.Biotoxin department of Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China 3.Graduate School of the Chinese Academy of Sciences, Beijing100009, China |
| 推荐引用方式 GB/T 7714 | Liu XH,You DW,Chen LH,et al. A novel bradykinin-like peptide from skin secretions of the frog, Rana nigrovittata[J]. JOURNAL OF PEPTIDE SCIENCE,2008,14(5):626-630. |
| APA | Liu XH,You DW,Chen LH,Wang X,Zhang KY,&Lai R[*].(2008).A novel bradykinin-like peptide from skin secretions of the frog, Rana nigrovittata.JOURNAL OF PEPTIDE SCIENCE,14(5),626-630. |
| MLA | Liu XH,et al."A novel bradykinin-like peptide from skin secretions of the frog, Rana nigrovittata".JOURNAL OF PEPTIDE SCIENCE 14.5(2008):626-630. |
入库方式： OAI收割
来源：昆明动物研究所
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