Two serine protease inhibitors from the skin secretions of the toad, Bombina microdeladigitora
文献类型：期刊论文
| 作者 | Lu XY1,3; Ma YF1,2; Wu J1,2; Lai R[*]1 |
| 刊名 | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
 |
| 出版日期 | 2008 |
| 卷号 | 149期号:4页码:608-612 |
| 关键词 | serine protease inhibitor amphibian Bombina microdeladigitora toad |
| ISSN号 | 1096-4959 |
| 通讯作者 | rlai@mail.kiz.ac.cn |
| 英文摘要 | Two serine protease inhibitors (named BMSI 1 and BMSI 2, respectively) were identified from the skin secretions of the toad, Bombina microdeladigitora. The cDNAs encoding BMSIs were cloned from a cDNA library prepared from the toad skin. The deduced complete amino acid sequences of BMSIs indicate that mature BMSI1 and BMSI2 are composed of 60 amino acids including 10 half-cystines to form 5 disulfide bridges. A FASTA search in the databanks revealed that BMSIs exhibit sequence similarity with other serine protease inhibitors from amphibians of the genus Bombina. BMSI1 potently inhibited trypsin and thrombin with a K(i) value of 0.02 mu M and 0.15 mu M, respectively. Sequence analysis revealed that all serine protease inhibitors from five amphibians of the genus Bombina share highly conserved primary structures. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by 2006BAD06B07 from the Ministry of Science and Technology of the People's Republic of China, KSCX2-YW-R-20 and KSCX2-YW-G-024 from Chinese Academy of Sciences. |
| 原文出处 | 2008149608.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/2545]  |
| 专题 | 昆明动物研究所_动物毒素室 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 |
| 作者单位 | 1.Biotoxin Units of Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China 2.Graduate School of the Chinese Academy of Sciences, Beijing100009, China 3.Department of Life Science & Technology, Changshu Institute of Technology, Changshu 215500, China |
| 推荐引用方式 GB/T 7714 | Lu XY,Ma YF,Wu J,et al. Two serine protease inhibitors from the skin secretions of the toad, Bombina microdeladigitora[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2008,149(4):608-612. |
| APA | Lu XY,Ma YF,Wu J,&Lai R[*].(2008).Two serine protease inhibitors from the skin secretions of the toad, Bombina microdeladigitora.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,149(4),608-612. |
| MLA | Lu XY,et al."Two serine protease inhibitors from the skin secretions of the toad, Bombina microdeladigitora".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 149.4(2008):608-612. |
入库方式： OAI收割
来源：昆明动物研究所
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