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A serine proteinase inhibitor from frog eggs with bacteriostatic activity

文献类型：期刊论文

作者Han YP1,2; Yu HN1; Yang XB3; Rees HH4; Liu JZ[*]1; Lai R[*]3
刊名COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
出版日期2008
卷号149期号:1页码:58-62
关键词serine proteinase inhibitor amphibian egg defensive Rana grahami
ISSN号1096-4959
通讯作者jzliu21@heinfo.net ; rlai@mail.kiz.ac.cn
合作状况其它
英文摘要By Sephadex G-50 gel filtration, Resource Q anionic exchange and C4 reversed phase liquid high performance liquid chromatography, a proteinase inhibitor protein (Ranaserpin) was identified and purified from the eggs of the odour frog, Rana grahami. The protein displayed a single band adjacent to the molecular weight marker of 14.4 kDa analyzed by SDS-PAGE. The inhibitor protein homogeneity and its molecular weight were confirmed again by MALDI-TOF mass spectrometry analysis. The MALDI-TOF mass spectrum analysis gave this inhibitor protein an m/z of 14422.26 that was matched well with the result from SDS-PAGE. This protein is a serine proteinase inhibitor targeting multiple proteinases including trypsin, elastase, and subtilisin. Ranaserpin inhibited the proteolytic activities of trypsin, elastase, and subtilisin. It has an inhibitory constant (K-i) of 6.2 x 10(-8) M, 2.7 x 10(-7) M and 2.2 x 10(-8) M for trypsin, elastase, and subtilisin, respectively. This serine proteinase inhibitor exhibited bacteriostatic effect on Gram-positive bacteria Bacillus subtilis (ATCC 6633). It was suggested that ranaserpin might act as a defensive role in resistance to invasion of pests or pathogens. This is the first report of serine proteinase inhibitor and its direct defensive role from amphibian eggs.
收录类别SCI
原文出处200814958.pdf
语种中文
公开日期2010-08-24
源URL[http://159.226.149.42:8088/handle/152453/2547]  
专题昆明动物研究所_动物毒素室
昆明动物研究所_动物模型与人类重大疾病机理重点实验室
作者单位1.College of Life Sciences School of Heibei Normal University, Shijiazhuang, Hebei, 050016 China
2.Department of Life Science & Technology, Changshu Institute of Technology, Changshu 215500, China
3.Biotoxins Units of Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China
4.School of Biological Sciences of University of Liverpool, Liverpool L69 7ZB, United Kingdom
推荐引用方式
GB/T 7714		 Han YP,Yu HN,Yang XB,et al. A serine proteinase inhibitor from frog eggs with bacteriostatic activity[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2008,149(1):58-62.
APA Han YP,Yu HN,Yang XB,Rees HH,Liu JZ[*],&Lai R[*].(2008).A serine proteinase inhibitor from frog eggs with bacteriostatic activity.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,149(1),58-62.
MLA Han YP,et al."A serine proteinase inhibitor from frog eggs with bacteriostatic activity".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 149.1(2008):58-62.

入库方式： OAI收割

来源：昆明动物研究所

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