Maximin 9, a novel free thiol containing antimicrobial peptide with antimycoplasma activity from frog Bombina maxima
文献类型：期刊论文
| 作者 | Lee WH; Zhang J; Zhang YX; Jin Y; Lai R; Zhang Y[*] |
| 刊名 | FEBS LETTERS
 |
| 出版日期 | 2005 |
| 卷号 | 579期号:20页码:4443-4448 |
| 关键词 | Amphibian Antimicrobial peptide cDNA sequence Free thiol Maximin Bombina maxima |
| ISSN号 | 0014-5793 |
| 通讯作者 | zhangy@mail.kiz.ac.en |
| 合作状况 | 其它 |
| 英文摘要 | Amphibian skin is a rich resource of antimicrobial peptides, like maximins and maximin Hs from frog Bombina maxima. Novel cDNA clones encoding a precursor protein, which comprises a novel maximin peptide (maximin 9) and reported maximin H3, were isolated from two constructed skin cDNA libraries of B. maxima. The predicted primary structure of maximin 9 is GIGRKFLGGVKTTFRCGVKDFASKHLY-NH2. A surprising substitution is at position 16, with a free cysteine in maximin 9 rather than usual conserved glycine in other reported maximins. Maximin 9, the homodimer form and its Cys16 to Gly16 mutant were synthesized and their antimicrobial activities were evaluated. Unlike previously reported maximin 3, the tested bacterial and fungal strains were resistant to maximin 9, its homodimer and the Cys16 to Gly16 mutant (with MICs>100 microM). On the other hand, interestingly, while eight clinical Mollicutes strains were generally resistant to maximin 9 homodimer and its Cys16 to Gly16 mutant, most of them are sensitive to maximin 9 at a peptide concentration of 30 microM, especially in the presence of dithiothreitol. These results indicate that the presence of a reactive Cys residue in maximin 9 is important for its antimycoplasma activity. The diversity of antimicrobial peptide cDNA structures encountered in B. maxima skin cDNA libraries and the antimicrobial specificity differences of the peptides may reflect well the species' adaptation to the unique microbial environments. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by ‘‘Wes- tern Light’’ Projects to Dr. Lee W.H and Dr. Zhang Y from The Chinese Academy of Sciences; and Grants from National Natural Sci- ence Foundation (30170195, 30200044, 30470380) and Yunnan Science and Technology Commission (2003C0066M). |
| 原文出处 | 20055794443.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/2673]  |
| 专题 | 昆明动物研究所_动物活性蛋白多肽组学 昆明动物研究所_动物毒素室 |
| 作者单位 | Department of Animal Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, 32 East Jiao Chang Road, Kunming, Yunnan 650223, PR China |
| 推荐引用方式 GB/T 7714 | Lee WH,Zhang J,Zhang YX,et al. Maximin 9, a novel free thiol containing antimicrobial peptide with antimycoplasma activity from frog Bombina maxima[J]. FEBS LETTERS,2005,579(20):4443-4448. |
| APA | Lee WH,Zhang J,Zhang YX,Jin Y,Lai R,&Zhang Y[*].(2005).Maximin 9, a novel free thiol containing antimicrobial peptide with antimycoplasma activity from frog Bombina maxima.FEBS LETTERS,579(20),4443-4448. |
| MLA | Lee WH,et al."Maximin 9, a novel free thiol containing antimicrobial peptide with antimycoplasma activity from frog Bombina maxima".FEBS LETTERS 579.20(2005):4443-4448. |
入库方式： OAI收割
来源：昆明动物研究所
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