Identification and molecular cloning of a novel neuromedin U analog from the skin secretions of toad Bombina maxima
文献类型：期刊论文
| 作者 | Lee WH1; Liu SB1,2; Shen JH1,3; Jin Y1; Lai R1; Zhang Y[*]1 |
| 刊名 | REGULATORY PEPTIDES
 |
| 出版日期 | 2005 |
| 卷号 | 129期号:1-3页码:43-47 |
| 关键词 | Neuropeptide Neuromedin U Amphibian Bombina maxima cDNA Sequence |
| ISSN号 | 0167-0115 |
| 通讯作者 | zhangy@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | Amphibian skin contains rich neuropeptides. In the present study, a novel neuromedin U (NmU) analog was isolated from skin secretions of Chinese red belly toad Bombina maxima. Being 17-amino acids long, its primary structure was established as DSSGIVGRPFFLFRPRN-NH2, in which the C-terminal 8-residue segment (FFLFRPRN) is the same as that of rat NmU, while the N-terminal part DSSGIVGRP shows a great sequence variation compared with those of NmU peptides from different resources. The peptide, named Bm-NmU-17, was found to elicit concentration-dependent contractile effects on smooth muscle of rat uterus horns. The cDNA structure of the peptide, as obtained by a 3'-RACE strategy and subsequently cloning from a skin cDNA library, was found to contain a coding region of 438 nucleotides. The encoded precursor is composed of 145 amino acids with a single copy of Bm-NmU-17 located towards the C-terminus. The sequence of the peptide is preceded by a dibasic site (Lys-Arg) and followed by the sequence of Gly-Arg-Lys, providing the sites of cleavage and releasing of the mature peptide. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by the grants of bWestern Light ProjectQ and bTenth Five Years PlanQ pre-research project from The Chinese Academy of Sciences; and grants from National Natural Science Foundation (30170195, 30200044, 30470380) and Yunnan Science and Technology Commis- sion (2001C0061M, 2001C0062M and 2003C0066M). |
| 原文出处 | 200512943.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/2675]  |
| 专题 | 昆明动物研究所_动物活性蛋白多肽组学 昆明动物研究所_动物毒素室 |
| 作者单位 | 1.Department of Animal Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, 32 East Jiao Chang Road, Kunming 650223, Yunnan, China 2.Graduate School of The Chinese Academy of Sciences, Beijing 100039, China 3.The First Affiliated Hospital of Kunming Medical College, Kunming, Yunnan 650032, China |
| 推荐引用方式 GB/T 7714 | Lee WH,Liu SB,Shen JH,et al. Identification and molecular cloning of a novel neuromedin U analog from the skin secretions of toad Bombina maxima[J]. REGULATORY PEPTIDES,2005,129(1-3):43-47. |
| APA | Lee WH,Liu SB,Shen JH,Jin Y,Lai R,&Zhang Y[*].(2005).Identification and molecular cloning of a novel neuromedin U analog from the skin secretions of toad Bombina maxima.REGULATORY PEPTIDES,129(1-3),43-47. |
| MLA | Lee WH,et al."Identification and molecular cloning of a novel neuromedin U analog from the skin secretions of toad Bombina maxima".REGULATORY PEPTIDES 129.1-3(2005):43-47. |
入库方式： OAI收割
来源：昆明动物研究所
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