Characterization and cloning of a novel phospholipase A(2) from the venom of Trimeresurus jerdonii snake
文献类型：期刊论文
| 作者 | Lu QM; Jin Y; Wei JF; Li DS; Zhu SW; Wang WY; Xiong YL[*] |
| 刊名 | TOXICON
 |
| 出版日期 | 2002 |
| 卷号 | 40期号:9页码:1313-1319 |
| 关键词 | Trimeresurus jerdonii Jerdoxin Phosphatidyl choline |
| ISSN号 | 0041-0101 |
| 通讯作者 | weiqinwq@yahoo.com.cn, wangwy@mail. kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | A phospholipase A(2) (PLA(2)), called jerdoxin, was isolated from Trimeresurus jerdonni snake venom and partially characterized. The protein was purified by three chromatographic steps. SDS-polyacrylamide gel electrophoresis in the presence or absence of dithiothreitol showed that it had a molecular mass of 15 kDa. Jerdoxin had an enzymatic activity of 39.4 micro mol/min/mg towards egg yolk phosphatidyl choline (PC). It induced edema in the footpads of mice. In addition, jerdoxin exhibited indirect hemolytic activity. About 97% hemolysis was observed when 2 micro g/ml enzyme was incubated for 90 min in the presence of PC and Ca(2+). No detectable hemolysis was noticed when PC was not added. Ca(2+) was necessary for jerdoxin to exert its hemolytic activity, since only 52% hemolysis was seen when Ca(2+) was absent in the reaction mixture. Furthermore, jerdoxin inhibited ADP induced rabbit platelet aggregation and the inhibition was dose dependent with an IC(50) of 1.0 micro M. The complete amino acid sequence of jerdoxin deduced from cDNA sequence shared high homology with other snake venom PLA(2)s, especially the D 49 PLA(2)s. Also, the residues concerned to Ca(2+) binding were conserved. This is the first report of cDNA sequence of T. jerdonii venom PLA(2). |
| 收录类别 | SCI |
| 原文出处 | 2002401313.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/2793]  |
| 专题 | 昆明动物研究所_其他 昆明动物研究所_动物毒素室 |
| 作者单位 | Department of Animal Toxicology, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, People’s Republic of China |
| 推荐引用方式 GB/T 7714 | Lu QM,Jin Y,Wei JF,et al. Characterization and cloning of a novel phospholipase A(2) from the venom of Trimeresurus jerdonii snake[J]. TOXICON,2002,40(9):1313-1319. |
| APA | Lu QM.,Jin Y.,Wei JF.,Li DS.,Zhu SW.,...&Xiong YL[*].(2002).Characterization and cloning of a novel phospholipase A(2) from the venom of Trimeresurus jerdonii snake.TOXICON,40(9),1313-1319. |
| MLA | Lu QM,et al."Characterization and cloning of a novel phospholipase A(2) from the venom of Trimeresurus jerdonii snake".TOXICON 40.9(2002):1313-1319. |
入库方式： OAI收割
来源：昆明动物研究所
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