Purification and cloning of a novel C-type lectin-like protein with platelet aggregation activity from Trimeresurus mucrosquamatus venom
文献类型：期刊论文
| 作者 | Wei Q1,2; Lu QM1; Jin Y1,2; Li R1; Wei JF1,2; Wang WY1; Xiong YL[*]1 |
| 刊名 | TOXICON
 |
| 出版日期 | 2002 |
| 卷号 | 40期号:9页码:1331-1338 |
| 关键词 | C-type lectin-like protein platelet aggregation heterodimer Trimeresurus mucrosquamatus |
| ISSN号 | 0041-0101 |
| 通讯作者 | weiqinwq@yahoo.com.cn |
| 合作状况 | 其它 |
| 英文摘要 | TMVA, a novel C-type lectin-like protein that induces platelet aggregation in a dose-dependent manner, was purified from the venom of Trimeresurus mucrosquamatus. It consists of two subunits, alpha (15,536 Da) and beta (14,873 Da). The mature amino acid sequences of the a (135 amino acids) and beta subunits (123 amino acids) were deduced from cloned cDNAs. Both of the sequences show great similarity to C-type lectin-like venom proteins, including a carbohydrate recognition domain. The cysteine residues of TMVA are conserved at positions corresponding to those of flavocetin-A and convulxin, including the additional Cys135 in the alpha subunit and Cys3 in the beta subunit. SDS-PAGE, mass spectrometry analysis and amino acid sequence showed that native TMVA exists as two convertible multimers Of (alphabeta)(2) and (alphabeta)(4) with molecular weights of 63,680 and 128,518 Da, respectively. The (alphabeta)(2) complex is stabilized by an interchain disulfide bridge between the two alphabeta-heterodimers, whereas the stabilization of the (alphabeta)(4) complex seems to involve non-covalent interactions between the (alphabeta)(2) complexes. |
| 收录类别 | SCI |
| 原文出处 | 2002401331.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/2795]  |
| 专题 | 昆明动物研究所_其他 昆明动物研究所_动物毒素室 |
| 作者单位 | 1.Department of Animal Toxicology, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, People’s Republic of China 2.Graduate School of the Chinese Academy of Science, People’s Republic of China |
| 推荐引用方式 GB/T 7714 | Wei Q,Lu QM,Jin Y,et al. Purification and cloning of a novel C-type lectin-like protein with platelet aggregation activity from Trimeresurus mucrosquamatus venom[J]. TOXICON,2002,40(9):1331-1338. |
| APA | Wei Q.,Lu QM.,Jin Y.,Li R.,Wei JF.,...&Xiong YL[*].(2002).Purification and cloning of a novel C-type lectin-like protein with platelet aggregation activity from Trimeresurus mucrosquamatus venom.TOXICON,40(9),1331-1338. |
| MLA | Wei Q,et al."Purification and cloning of a novel C-type lectin-like protein with platelet aggregation activity from Trimeresurus mucrosquamatus venom".TOXICON 40.9(2002):1331-1338. |
入库方式： OAI收割
来源：昆明动物研究所
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