Actions of two serine proteases from Trimeresurus jerdonii venom on chromogenic substrates and fibrinogen
文献类型：期刊论文
| 作者 | Jin Y1,2; Lu QM1; Wang WY1; Xiong YL[*]1 |
| 刊名 | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY Part B
 |
| 出版日期 | 2002 |
| 卷号 | 132期号:3页码:529-534 |
| 关键词 | chromogenic substrates fibrinogen fibrinopeptide snake venom serine protease thrombin-like enzyme Trimeresurus jerdonii |
| ISSN号 | 1096-4959 |
| 通讯作者 | luqiumin@hotmail.com |
| 合作状况 | 其它 |
| 英文摘要 | Jerdonobin and jerdofibrase are two serine proteases purified from the venom of Trimeresurus jerdonii. The Michaelis constant K-m and the catalytic rate constant K-cat of jerdonobin or jerdofibrase on three chromogenic substrates, H-D-Pro-Phe-Arg-pNA (S2302), H-D-Phe-pipecolyl-Arg-pNA (S2238), and H-D-Val-Leu-Lys-pNA (S2251) were obtained from lineweaver-Burk plots. Jerdofibrase could hydrolyze all three substrates, but jerdonobin had no detectable activity on S2251, suggesting a relatively broader substrate specificity for jerdofibrase than jerdonobin. By SDS-PAGE, jerdofibrase preferentially degraded Bbeta-chain of fibrinogen. It also degraded Aalpha-chain of fibrinogen with relatively slow activity, but did not act on the gamma-chain. In contrast, jerdonobin did not degrade fibrinogen within 12 h. Fibrinopeptides liberation test, identified by HPLC, showed jerdonobin released fibrinopeptide A and a small amount of fibrinopeptide B. Unlike jerdonobin, jerdofibrase mainly released fibrinopeptide B. These results indicate that the two enzymes differ in their ability to hydrolyze chromogenic substrates and in their actions on fibrinogen. |
| 收录类别 | SCI |
| 原文出处 | 2002132529.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/2799]  |
| 专题 | 昆明动物研究所_其他 昆明动物研究所_动物毒素室 |
| 作者单位 | 1.Department of Animal Toxinology, Kunming Institute of Zoology, Kunming 650223, Yunnan, PR China a 2.Graduate School of Chinese Academy of Sciences, Yunnan, PR China |
| 推荐引用方式 GB/T 7714 | Jin Y,Lu QM,Wang WY,et al. Actions of two serine proteases from Trimeresurus jerdonii venom on chromogenic substrates and fibrinogen[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY Part B,2002,132(3):529-534. |
| APA | Jin Y,Lu QM,Wang WY,&Xiong YL[*].(2002).Actions of two serine proteases from Trimeresurus jerdonii venom on chromogenic substrates and fibrinogen.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY Part B,132(3),529-534. |
| MLA | Jin Y,et al."Actions of two serine proteases from Trimeresurus jerdonii venom on chromogenic substrates and fibrinogen".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY Part B 132.3(2002):529-534. |
入库方式： OAI收割
来源：昆明动物研究所
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