A novel short neurotoxin, cobrotoxin c, from monocellate cobra (Naja kaouthia) venom: isolation and purification, primary and secondary structure determination, and tertiary structure modeling
文献类型：期刊论文
| 作者 | Meng QX; Wang WY; Lu QM; Jin Y; Wei JF; Zhu SW; Xiong YL[*] |
| 刊名 | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
 |
| 出版日期 | 2002 |
| 卷号 | 132期号:1页码:113-121 |
| 关键词 | snake venom short neurotoxin Naja kaouthia cobrotoxin c amino acid sequence three-finger proteins lethal toxicity comparative structure functional domain |
| ISSN号 | 1532-0456 |
| 通讯作者 | qx_meng@hotmail.com |
| 合作状况 | 其它 |
| 英文摘要 | A novel short neurotoxin, cobrotoxin c (CBT C) was isolated from the venom of monocellate cobra (Naja kaouthia) using a combination of ion-exchange chromatography and FPLC. Its primary structure was determined by Edman degradation. CBT C is composed of 61 amino acid residues. It differs from cobrotoxin b (CBT B) by only two amino acid substitutions, Thr/Ala11 and Arg/Thr56, which are not located on the functionally important regions by sequence similarity. However, the LD50 is 0.08 mg/g to mice, i.e. approximately five-fold higher than for CBT B. Strikingly, a structure-function relationship analysis suggests the existence of a functionally important domain on the outside of Loop III of CBT C. The functionally important basic residues on the outside of Loop III might have a pairwise interaction with alpha subunit, instead of gamma or delta subunits of the nicotinic acetylcholine receptor (nAChR). |
| 收录类别 | SCI |
| 资助信息 | The project was supported by the National Nature Science Fund of China (Project number: 39670165). |
| 原文出处 | 2002132113.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/2801]  |
| 专题 | 昆明动物研究所_其他 昆明动物研究所_动物毒素室 |
| 作者单位 | Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, PR China |
| 推荐引用方式 GB/T 7714 | Meng QX,Wang WY,Lu QM,et al. A novel short neurotoxin, cobrotoxin c, from monocellate cobra (Naja kaouthia) venom: isolation and purification, primary and secondary structure determination, and tertiary structure modeling[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,2002,132(1):113-121. |
| APA | Meng QX.,Wang WY.,Lu QM.,Jin Y.,Wei JF.,...&Xiong YL[*].(2002).A novel short neurotoxin, cobrotoxin c, from monocellate cobra (Naja kaouthia) venom: isolation and purification, primary and secondary structure determination, and tertiary structure modeling.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,132(1),113-121. |
| MLA | Meng QX,et al."A novel short neurotoxin, cobrotoxin c, from monocellate cobra (Naja kaouthia) venom: isolation and purification, primary and secondary structure determination, and tertiary structure modeling".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY 132.1(2002):113-121. |
入库方式： OAI收割
来源：昆明动物研究所
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