A novel disintegrin, jerdonatin, inhibits platelet aggregation and sperm-egg binding
文献类型：期刊论文
| 作者 | Zhou XD1,2,3; Ding CH1,2; Tai H1; Jin Y1; Chen RQ1,2; Lu QM1; Wang WY1; Xiong YL[*]1 |
| 刊名 | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
 |
| 出版日期 | 2004 |
| 卷号 | 139期号:1页码:117-122 |
| 关键词 | Venom Trimeresurus jerdonii cDNA Sequence Peptide Disintegrin Platelet Sperm–egg binding Fertilization |
| ISSN号 | 1096-4959 |
| 通讯作者 | toxinlab@mail.kiz.ac.cn |
| 英文摘要 | A novel disintegrin, jerdonatin, was purified to homogeneity from Trimeresurus jerdonii venom by gel filtration and reversed-phase high-pressure liquid chromatography. We isolated the cDNA encoding jerdonatin from the snake venom gland. Jerdonatin cDNA precursor encoded pre-peptide, metalloprotease and disintegrin domain. Jerdonatin is composed of 72 amino acid residues including 12 cysteines and the tripeptide sequence Arg-Gly-Asp (RGD), a well-known characteristic of the disintegrin family. Molecular mass of jerdonatin was determined to be 8011 Da by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS). Jerdonatin inhibited ADP- and collagen-induced human platelet aggregation with IC50 of 123 and 135 nM, respectively. We also investigated the effect of jerdonatin on the binding of B6D2F1 hybrid mice spermatozoa to mice zona-free eggs and their subsequent fusion. Jerdonatin significantly inhibited sperm-egg binding in a concentration-dependent manner, but had no effect on the fusion of sperm-egg. These results indicate that integrins on the egg play a role in mammalian fertilization. |
| 收录类别 | SCI |
| 资助信息 | This research was supported by the fund from Natural Science Foundation of Yunnan Science and Technology Committee, No. 2002C0063M. |
| 原文出处 | 2004139117.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/2805]  |
| 专题 | 昆明动物研究所_其他 昆明动物研究所_动物毒素室 |
| 作者单位 | 1.Department of Animal Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, No. 32 Jiaochangdonglu, Kunming 650223, Yunnan, PR China 2.Graduate School of the Chinese Academy of Sciences, Beijing 100080, PR China 3.Faculty of Biological and Chemical Engineering, Kumming University of Science and Technology, Kunming 650224, Yunnan, PR China |
| 推荐引用方式 GB/T 7714 | Zhou XD,Ding CH,Tai H,et al. A novel disintegrin, jerdonatin, inhibits platelet aggregation and sperm-egg binding[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2004,139(1):117-122. |
| APA | Zhou XD.,Ding CH.,Tai H.,Jin Y.,Chen RQ.,...&Xiong YL[*].(2004).A novel disintegrin, jerdonatin, inhibits platelet aggregation and sperm-egg binding.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,139(1),117-122. |
| MLA | Zhou XD,et al."A novel disintegrin, jerdonatin, inhibits platelet aggregation and sperm-egg binding".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 139.1(2004):117-122. |
入库方式： OAI收割
来源：昆明动物研究所
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