A novel high molecular weight metalloproteinase cleaves fragment F1 of activated human prothrombin
文献类型：期刊论文
| 作者 | Chen RQ1,2; Jin Y1; Wu JB1,2; Zhou XD1,2; Li DS1; Lu QM1; Wang WY1; Xiong YL[*]1 |
| 刊名 | TOXICON
 |
| 出版日期 | 2004 |
| 卷号 | 44期号:3页码:281-287 |
| 关键词 | Hemorrhage SVMP Trimeresurus jerdonii Fibrinogenolytic activity Activation fragment F1 |
| ISSN号 | 0041-0101 |
| 通讯作者 | chenrunqiang@hotmail.com |
| 合作状况 | 其它 |
| 英文摘要 | A hemorrhagic proteinase, jerdohagin, was purified from Trimeresurus jerdonii venom by gel filtration and ion-exchange chromatographies. It was a single chain polypeptide with an apparent molecular weight of 96 kDa as estimated by SDS-PAGE under the non-reducing and reducing conditions. Internal peptide sequencing indicated that it consisted of metalloproteinase, disintegrin-like and cysteine-rich domains and belonged to the class III snake venom metalloproteinases (class P-III SVMPs). Like other typical metalloproteinases, hemorrhagic activities of jerdohagin were completely inhibited by EDTA, but not by PMSF. Jerdohagin preferentially degraded alpha-chain of human fibrinogen. Interestingly, jerdohagin did not activate human prothrombin, whereas it cleaved human prothrombin and fragment F1 of activated human prothrombin. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by Natural Science Foundation of Yunnan Science and Technology Committee (No.2002C0063M). |
| 原文出处 | 200444281.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/2807]  |
| 专题 | 昆明动物研究所_其他 昆明动物研究所_动物毒素室 |
| 作者单位 | 1.Department of Animal Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming 650223, China 2.Graduate School of The Chinese Academy of Sciences, Beijing 100039, China |
| 推荐引用方式 GB/T 7714 | Chen RQ,Jin Y,Wu JB,et al. A novel high molecular weight metalloproteinase cleaves fragment F1 of activated human prothrombin[J]. TOXICON,2004,44(3):281-287. |
| APA | Chen RQ.,Jin Y.,Wu JB.,Zhou XD.,Li DS.,...&Xiong YL[*].(2004).A novel high molecular weight metalloproteinase cleaves fragment F1 of activated human prothrombin.TOXICON,44(3),281-287. |
| MLA | Chen RQ,et al."A novel high molecular weight metalloproteinase cleaves fragment F1 of activated human prothrombin".TOXICON 44.3(2004):281-287. |
入库方式： OAI收割
来源：昆明动物研究所
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