A unique group of inactive serine protease homologues from snake venom
文献类型：期刊论文
| 作者 | Wu JB1,2; Jin Y1; Zhong SR3; Chen RQ1; Zhu SM1; Wang WY1; Lu QM[*]1; Xiong YL1 |
| 刊名 | TOXICON
 |
| 出版日期 | 2008 |
| 卷号 | 52期号:2页码:277-284 |
| 关键词 | snake venom serine protease homologue phylogenetic analysis Trimeresurus jerdonii |
| ISSN号 | 0041-0101 |
| 通讯作者 | lvqm@mail.kiz.ac.cn |
| 英文摘要 | A number of inactive serine protease homologues (SPHs), which have poorly understood functions, have been identified in invertebrates and vertebrates. Recently, several SPH transcripts have been reported from snake venom glands, which provide potential new tools for the study of the functions of SPHs. Herein we report for the first time a snake venom serine protease homologue (svSPH) protein, designated as TjsvSPH, isolated from the venom of Tilmeresurus jerdonii. Despite its high sequence similarity to snake venom serine proteases (SVSPs), TjsvSPH is devoid of arginine esterase and proteolytic activity. This is probably due to the replacement of Arg-43 by His-43 in the catalytic triad. TjsvSPH did not influence the coagulation time of human plasma, induce human platelet aggregation, inhibit adenosine diphosphate/thrombin-induced human platelet aggregation or increase capillary permeability. Phylogenetic analysis showed that svSPHs were separated from SVSPs and formed an independent group. Structural analysis revealed that the structures of svSPHs are quite different from those of SPHs previously reported. These results indicate that snake venoms contain a unique group of svSPH proteins. (C) 2008 Elsevier Ltd. All rights reserved. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by Western Light Project of CAS, Chinese National Natural Science Foundation (30770431), and Yunnan Natural Science Foundation (2007C102M). |
| 原文出处 | 200852277.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/2821]  |
| 专题 | 昆明动物研究所_其他 昆明动物研究所_动物毒素室 |
| 作者单位 | 1.Department of Animal Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, 32 Jiaochang East Road, Kunming 650223, PR China 2.Graduate School of The Chinese Academy of Sciences, Beijing 100080, PR China 3.Department of Forensic Biology, Faculty of Forensic Medicine, Kunming Medical College, Kunming 650031, PR China |
| 推荐引用方式 GB/T 7714 | Wu JB,Jin Y,Zhong SR,et al. A unique group of inactive serine protease homologues from snake venom[J]. TOXICON,2008,52(2):277-284. |
| APA | Wu JB.,Jin Y.,Zhong SR.,Chen RQ.,Zhu SM.,...&Xiong YL.(2008).A unique group of inactive serine protease homologues from snake venom.TOXICON,52(2),277-284. |
| MLA | Wu JB,et al."A unique group of inactive serine protease homologues from snake venom".TOXICON 52.2(2008):277-284. |
入库方式： OAI收割
来源：昆明动物研究所
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