L-amino acid oxidase from Naja atra venom activates and binds to human platelets
文献类型：期刊论文
| 作者 | Li R1; Zhu SW1; Wu JB1; Wang WY1; Lu QM[*]1; Clemetson KJ2 |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2008 |
| 卷号 | 40期号:1页码:19-26 |
| 关键词 | L-amino acid oxidase Naja atra platelet hydrogen peroxide |
| ISSN号 | 1672-9145 |
| 通讯作者 | lvqm@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | An L-amino acid oxidase (LAAO), NA-LAAO, was purified from the venom of Naja atra. Its N-terminal sequence shows great similarity with LAAOs from other snake venoms. NA-LAAO dose-dependently induced aggregation of washed human platelets. However, it had no activity on platelets in platelet-rich plasma. A low concentration of NA-LAAO greatly promoted the effect of hydrogen peroxide, whereas hydrogen per oxide itself had little activation effect on plate lets. NA-LAAO induced tyrosine phosphorylation of a number of platelet proteins including Src kinase, spleen tyrosine kinase, and phospholipase C gamma 2. Unlike convulxin, Fc receptor gamma chain and T lymphocyte adapter protein are not phosphorylated in NA-LAAO-activated platelets, suggesting an activation mechanism different from the glycoprotein VI pathway. Catalase inhibited the platelet aggregation and platelet protein phosphorylation induced by NA-LAAO. NA-LAAO bound to fixed platelets as well as to platelet lysates of Western blots. Furthermore, affinity chromatography of platelet proteins on an NA-LAAO-Sepharose 4B column isolated a few platelet membrane proteins, suggesting that binding of NA-LAAO to the platelet membrane might play a role in its action on platelets. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by the grants from the Natural Science Foundation of China (30770431), the Natural Science Foundation of Yunnan Science and Technology Committee, Yunnan Province (2007C102M) and the Western Light Project of Chinese Academy of Sciences |
| 原文出处 | 20084019.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/2823]  |
| 专题 | 昆明动物研究所_动物毒素室 昆明动物研究所_其他 |
| 作者单位 | 1.Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 2.Theodor Kocher Institute, University of Berne, Berne CH-3012, Switzerland |
| 推荐引用方式 GB/T 7714 | Li R,Zhu SW,Wu JB,et al. L-amino acid oxidase from Naja atra venom activates and binds to human platelets[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2008,40(1):19-26. |
| APA | Li R,Zhu SW,Wu JB,Wang WY,Lu QM[*],&Clemetson KJ.(2008).L-amino acid oxidase from Naja atra venom activates and binds to human platelets.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,40(1),19-26. |
| MLA | Li R,et al."L-amino acid oxidase from Naja atra venom activates and binds to human platelets".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 40.1(2008):19-26. |
入库方式： OAI收割
来源：昆明动物研究所
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