A Highly Active Anticomplement Factor from the Venom of Naja kaouthia
文献类型：期刊论文
| 作者 | Sun QY; Lu QM; Wang WY; Xiong YL[*] |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2001 |
| 卷号 | 33期号:5页码:483-488 |
| 关键词 | anticomplement factor complement venom |
| ISSN号 | 0582-9879 |
| 其他题名 | 一个高活性的盂加拉眼镜蛇毒抗补体因子 |
| 通讯作者 | sunqy@hotmail. com |
| 合作状况 | 其它 |
| 中文摘要 | 用离子交换层析和分子筛从云南南部产的盂加拉眼镜蛇（Naja kaouthia)蛇毒中分离到一个高活性的抗补体因子（anticomplement factor)。它表现出较强的体内、体外抗补体活性，其抗补体活性的比活力为1515u/mg，纯化的抗补体因子在聚丙烯酰胺凝胶电泳中呈现一条带，经SDS-PAGE，确定其全分子量为149kD，还原性SDS-PAGE表明，它由3条多肽链共价结合而成，3条多肽链分子量分别为65.4kD、52.1kD和35.5kD。最小的一条多肽链在还原条件下呈现多态性，一般可见两条带（35.5kD和33.7kD)。过碘酸席夫试剂染色表明，其3条多肽链均含有糖。定量测定表明中性糖含量为1.78%，唾液酸含量为0.38%，其等电点为6.2。对其氨基酸组成分析表明它含有较多的酸性氨基酸，对其3条多肽链的N末端氨基酸序列进行了测定。 |
| 英文摘要 | A highly active anticomplement factor (cobra venom factor) from the venom of Naja kaouthia in South Yunnan, China was isolated by sequential column chromatography (SP-Sephadex-C25, Q Sepharose HP and Sephadex G-150). It displays strong anticomplement activities in vitro and in vivo, and has anticomplement activity of 1 515 u per mg. The purified anticomplement factor was homogeneous on SDS-PAGE with a molecular weight of 149 kD. It is composed of three polypeptide chains which are connected by disulfide bonds. The molecular weights of the three chains were determined to be 65. 4 kD (alpha -chain), 52. 1 kD (beta -chain), and 35. 5 kD (gamma -chain). The gamma -chain displayed size heterogeneity, and two bands could be clearly detected in gels. All polypeptide chains were stained with periodate-Schiff reagent, suggesting the presence of carbohydrate. Neutral sugar and sialic acid were determined to be 1. 78 % and 0. 38 %, respectively. The isoelectric point of this anticomplement factor was 6. 2. Its amino acid composition was analyzed and N-terminal amino acid sequence of each polypeptide chain was determined. |
| 收录类别 | 其他 |
| 资助信息 | This work was supported by a grant from the Key Research Pro 2 grams of Yunnan Province , China (No. C0007Z) |
| 原文出处 | 2001335483.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/3795]  |
| 专题 | 昆明动物研究所_动物毒素室 昆明动物研究所_其他 |
| 作者单位 | Kunming Institute of Zoology , the Chinese Academy of Sciences , Kunming 650223 , China |
| 推荐引用方式 GB/T 7714 | Sun QY,Lu QM,Wang WY,et al. A Highly Active Anticomplement Factor from the Venom of Naja kaouthia[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2001,33(5):483-488. |
| APA | Sun QY,Lu QM,Wang WY,&Xiong YL[*].(2001).A Highly Active Anticomplement Factor from the Venom of Naja kaouthia.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,33(5),483-488. |
| MLA | Sun QY,et al."A Highly Active Anticomplement Factor from the Venom of Naja kaouthia".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 33.5(2001):483-488. |
入库方式： OAI收割
来源：昆明动物研究所
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