Purification and cloning of cysteine-rich proteins from Trimeresurus jerdonii and Naja atra venoms
文献类型：期刊论文
| 作者 | Jin Y; Lu QM; Zhou XD; Zhu SW; Li R; Wang WY; Xiong YL[*] |
| 刊名 | TOXICON
 |
| 出版日期 | 2003 |
| 卷号 | 42期号:5页码:539-547 |
| 关键词 | Trimeresurus jerdonii Naja atra Cysteine-rich venom protein |
| ISSN号 | 0041-0101 |
| 通讯作者 | wangwy@mail.kiz.ac.cn ; jinyangkiz@hotmail.com |
| 合作状况 | 其它 |
| 英文摘要 | Three 26 kDa proteins, named as TJ-CRVP, NA-CRVP1 and NA-CRVP2, were isolated from the venoms of Trimeresurus jerdonii and Naja atra, respectively. The N-terminal sequences of TJ-CRVP and NA-CRVPs were determined. These components were devoid of the enzymatic activities tested, such as phospholipase A(2), arginine esterase, proteolysis, L-amino acid oxidase, 5'nucleotidase, acetylcholinesterase. Furthermore, these three components did not have the following biological activities: coagulant and anticoagulant activities, lethal activity, myotoxicity, hemorrhagic activity, platelet aggregation and platelet aggregation-inhibiting activities. These proteins are named as cysteine-rich venom protein (CRVP) because their sequences showed high level of similarity with mammalian cysteine-rich secretory protein (CRISP) family. Recently, some CRISP-like proteins were also isolated from several different snake venoms, including Agkistrodon blomhoffi, Trimeresurus flavoviridis, Lanticauda semifascita and king cobra. We presumed that CRVP might be a common component in snake venoms. Of particular interest, phylogenetic analysis and sequence alignment showed that NA-CRVP1 and ophanin, both from elapid snakes, share higher similarity with CRVPs from Viperidae snakes. |
| 收录类别 | SCI |
| 资助信息 | This work was granted by Natural Science Foundation of Yunnan Province, China (Grant No. 2002C0063M). |
| 原文出处 | 200342539.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/5265]  |
| 专题 | 昆明动物研究所_动物毒素室 昆明动物研究所_其他 |
| 作者单位 | Department of Animal Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming, Yunnan 650223, People’s Republic of China |
| 推荐引用方式 GB/T 7714 | Jin Y,Lu QM,Zhou XD,et al. Purification and cloning of cysteine-rich proteins from Trimeresurus jerdonii and Naja atra venoms[J]. TOXICON,2003,42(5):539-547. |
| APA | Jin Y.,Lu QM.,Zhou XD.,Zhu SW.,Li R.,...&Xiong YL[*].(2003).Purification and cloning of cysteine-rich proteins from Trimeresurus jerdonii and Naja atra venoms.TOXICON,42(5),539-547. |
| MLA | Jin Y,et al."Purification and cloning of cysteine-rich proteins from Trimeresurus jerdonii and Naja atra venoms".TOXICON 42.5(2003):539-547. |
入库方式： OAI收割
来源：昆明动物研究所
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