A new protein structure of P-II class snake venom metalloproteinases: it comprises metalloproteinase and disintegrin domains
文献类型：期刊论文
| 作者 | Chen RQ1,2; Jin Y1; Wu JB1,2; Zhou XD1,2; Lu QM1; Wang WY1; Xiong YL[*]1 |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2003 |
| 卷号 | 310期号:1页码:182-187 |
| 关键词 | SVMP Disintegrin Platelet aggregation Phylogenetic analysis Trimeresurus jerdonii |
| ISSN号 | 0006-291X |
| 通讯作者 | chenrunqiang@hotmail.com |
| 合作状况 | 其它 |
| 英文摘要 | A new metalloproteinase-disintegrin, named Jerdonitin, was purified from Trimeresurus jerdonii venom with a molecular weight of 36 kDa on SDS-PAGE. It dose-dependently inhibited ADP-induced human platelet aggregation with IC(50) of 120nM. cDNA cloning and sequencing revealed that Jerdonitin belonged to the class II of snake venom metalloproteinases (SVMPs) (P-II class). Different from other P-II class SVMPs, metalloproteinase and disintegrin domains of its natural protein were not separated, confirmed by internal peptide sequencing. Compared to other P-II class SVMPs, Jerdonitin has two additional cysteines (Cys219 and Cys238) located in the spacer domain and disintegrin domain, respectively. They probably form a disulfide bond and therefore the metalloproteinase and disintegrin domains cannot be separated by posttranslationally processing. In summary, comparison of the amino acid sequences of Jerdonitin with those of other P-II class SVMPs by sequence alignment and phylogenetic analysis, in conjunction with natural protein structure data, suggested that it was a new type of P-II class SVMPs. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by Natural Science Foundation of Yunnan Science and Technology Committee (No. 2002C0063M). |
| 原文出处 | 2003310182.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/5267]  |
| 专题 | 昆明动物研究所_动物毒素室 昆明动物研究所_其他 |
| 作者单位 | 1.Department of Animal Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming 650223, PR China 2.Graduate School of The Chinese Academy of Sciences, Beijing 100080, PR China |
| 推荐引用方式 GB/T 7714 | Chen RQ,Jin Y,Wu JB,et al. A new protein structure of P-II class snake venom metalloproteinases: it comprises metalloproteinase and disintegrin domains[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2003,310(1):182-187. |
| APA | Chen RQ.,Jin Y.,Wu JB.,Zhou XD.,Lu QM.,...&Xiong YL[*].(2003).A new protein structure of P-II class snake venom metalloproteinases: it comprises metalloproteinase and disintegrin domains.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,310(1),182-187. |
| MLA | Chen RQ,et al."A new protein structure of P-II class snake venom metalloproteinases: it comprises metalloproteinase and disintegrin domains".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 310.1(2003):182-187. |
入库方式： OAI收割
来源：昆明动物研究所
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