Purification, characterization and primary structure of a chymotrypsin inhibitor from Naja atra venom
文献类型：期刊论文
| 作者 | Zhou XD1,2,3; Jin Y1; Lu QM1; Li DS1; Zhu SW1; Wang WY1; Xiong YL[*]1 |
| 刊名 | Comparative Biochemistry and Physiology Part B
 |
| 出版日期 | 2004 |
| 卷号 | 137期号:2页码:219-224 |
| 关键词 | Amino acid sequence Chinese cobra Chymotrypsin inhibitor Isolation Naja atra Snake venom |
| ISSN号 | 1096-4959 |
| 通讯作者 | toxinlab@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | A chymotrypsin inhibitor, designated NA-CI, was isolated from the venom of the Chinese cobra Naja atra by three-step chromatography. It inhibited bovine alpha-chymotrypsin with a Ki of 25 nM. The molecular mass of NA-CI was determined to be 6403.8 Da by matrix-assisted laser-desorption ionization time-of-flight (MALDI-TOF) analysis. The complete amino acid sequence was determined after digestion of S-carboxymethylated inhibitor with Staphylococcus aureus V8 protease and porcine trypsin. NA-CI was a single polypeptide chain composed of 57 amino acid residues. The main contact site with the protease (P1) has a Phe, showing the specificity of the inhibitor. NA-CI shared great similarity with the chymotrypsin inhibitor from Naja naja venom (identities=89.5%) and other snake venom protease inhibitors. |
| 收录类别 | SCI |
| 资助信息 | This research was supported by Natural Science Foundation of Yunnan Science and Technology Committee, No. 2002C0063M. |
| 原文出处 | 2004137219.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/5317]  |
| 专题 | 昆明动物研究所_其他 昆明动物研究所_动物毒素室 |
| 作者单位 | 1.Department of Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, 650223 Kunming, Yunnan, PR China 2.Graduate School of the Chinese Academy of Sciences, 100039 Beijing, PR China 3.Faculty of Biological and Chemical Engineering, Kunming University of Science and Technology, 650224 Kunming, Yunnan |
| 推荐引用方式 GB/T 7714 | Zhou XD,Jin Y,Lu QM,et al. Purification, characterization and primary structure of a chymotrypsin inhibitor from Naja atra venom[J]. Comparative Biochemistry and Physiology Part B,2004,137(2):219-224. |
| APA | Zhou XD.,Jin Y.,Lu QM.,Li DS.,Zhu SW.,...&Xiong YL[*].(2004).Purification, characterization and primary structure of a chymotrypsin inhibitor from Naja atra venom.Comparative Biochemistry and Physiology Part B,137(2),219-224. |
| MLA | Zhou XD,et al."Purification, characterization and primary structure of a chymotrypsin inhibitor from Naja atra venom".Comparative Biochemistry and Physiology Part B 137.2(2004):219-224. |
入库方式： OAI收割
来源：昆明动物研究所
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