Molecular characterization of a weak fibrinogen-clotting enzyme from Trimeresurus jerdonii venom
文献类型：期刊论文
| 作者 | Jin Y; Lu QM; Chen RQ; Wu JB; Xiong YL[*] |
| 刊名 | TOXICON
 |
| 出版日期 | 2005 |
| 卷号 | 45期号:3页码:353-360 |
| 关键词 | Snake venom Thrombin Plasminogen Fibrinogen Trimeresurus jerdonii |
| ISSN号 | 0041-0101 |
| 通讯作者 | jinyang@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | A fibrinogen-clotting enzyme designed as jerdonobin-II was isolated from the venom of Trimeresurus jerdonii. It differed in molecular weight and N-terminal sequence with the previously isolated jerdonobin, a thrombin-like enzyme from the same venom. The enzyme consists of a single polypeptide chain with molecular weights of 30,000 and 32,000 under non-reducing and reducing conditions, respectively. Jerdonobin-II showed weak fibrinogen clotting activity and its activity unit on fibrinogen was calculated to be less than one unit using human thrombin as standard. The precursor protein sequence of jerodonobin-II was deduced from cloned cDNA sequence. The sequence shows high similarity (identity=89%) to TSV-PA, a specific plasminogen activator from venom of T. stejnegeri. Despite of the sequence similarity, jerdonobin-II was found devoid of plasminogen activating effect. Sequence alignment analysis suggested that the replacement of Lys239 in TSV-PA to Gln239 in jerdonobin-II might play an important role on their plasminogen activating activity difference. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by the grants of 2001C0062M and 2003C0066M from Yunnan Science and Technology Commission. |
| 原文出处 | 200545353.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/5349]  |
| 专题 | 昆明动物研究所_动物毒素室 昆明动物研究所_其他 |
| 作者单位 | Department of Animal Toxinology, Kunming Institute of Zoology, the Chinese Academy of Sciences, East Jiao-Chang Road, Kunming 650223, Yunnan, People’s Republic of China |
| 推荐引用方式 GB/T 7714 | Jin Y,Lu QM,Chen RQ,et al. Molecular characterization of a weak fibrinogen-clotting enzyme from Trimeresurus jerdonii venom[J]. TOXICON,2005,45(3):353-360. |
| APA | Jin Y,Lu QM,Chen RQ,Wu JB,&Xiong YL[*].(2005).Molecular characterization of a weak fibrinogen-clotting enzyme from Trimeresurus jerdonii venom.TOXICON,45(3),353-360. |
| MLA | Jin Y,et al."Molecular characterization of a weak fibrinogen-clotting enzyme from Trimeresurus jerdonii venom".TOXICON 45.3(2005):353-360. |
入库方式： OAI收割
来源：昆明动物研究所
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