Jerdonuxin, a novel snaclec (snake C-type lectin) with platelet aggregation activity from Trimeresurus jerdonii venom
文献类型：期刊论文
| 作者 | Chen ZM1,2; Wu JB3; Zhang Y1; Yu GY1; Lee WH1; Lu QM[*]1; Zhang Y[*]1 |
| 刊名 | TOXICON
 |
| 出版日期 | 2011 |
| 卷号 | 57期号:1页码:109-116 |
| 关键词 | Jerdonuxin Purification Molecular cloning Platelet aggregation Glycoprotein Ib |
| 通讯作者 | lvqm@mail.kiz.ac.cn ; zhangy@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | Serious clinical symptoms of Trimeresurus jerdonii bite are mainly caused by abnormalities of blood system. We have previously identified and characterized several bioactive components affecting human blood system, such as serine proteases, metalloproteinases and dis-integrins. But few snaclec was characterized in the I jerdonii venom. In this study, a novel snaclec, named jerdonuxin, was isolated, molecular cloned and characterized as a human platelet agonist. On SDS-polyacrylamide gel electrophoresis, jerdonuxin showed a single band with an apparent molecular weight of 120 kDa under non-reducing conditions and two distinct bands with apparent molecular weights of 18 kDa (alpha-subunit) and 14 kDa (beta-subunit) under reducing conditions. The cDNA sequence of each subunit of jerdonuxin was identified. The precursors of both subunits contain a 23-amino acid residue signal peptide and the mature proteins are composed of 135 and 125 amino acids for alpha- and beta-subunits, respectively. The N-terminal amino acid sequences of each subunit determined by Edman degradation were consistent with deduced amino acid sequences of cDNA. Jerdonuxin dose-dependently induced human platelet aggregation. The phosphorylation profile pattern induced by jerdonuxin showed similar with mucetin (a platelet agonist via glycoprotein Ib), but different from stejnulxin (an agonist via glycoprotein VI). The jerdonuxin-induced platelet aggregation was inhibited by the anti-GPIb alpha or anti-GPIIb polyclonal antibodies, but not by anti-GPVI polyclonal antibodies. In summary, a novel snaclec of platelet agonist was purified and characterized from the T. jerdonii venom and our data also suggested that GPIb was involved in jerdonuxin-induced platelet aggregation. (C) 2010 Elsevier Ltd. All rights reserved |
| 收录类别 | SCI |
| 资助信息 | This work was supported by grants from the National Basic Research Program of China (973 Program, 2010CB529800), the Chinese National Natural Science Foundation (30630014, 30770431 and 30670412) and the Chinese Academy of Sciences “Key Research Direction” (KSCX2-YW-R-088, KSCX2-YW-R-212), the Western Light Project of CAS, Chinese National Natural Science Founda- tion (30770431), Yunnan Natural Science Foundation (2007C102M). |
| 语种 | 英语 |
| 公开日期 | 2011-03-21 |
| 源URL | [http://159.226.149.42:8088/handle/353002/6589]  |
| 专题 | 昆明动物研究所_动物活性蛋白多肽组学 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 昆明动物研究所_动物毒素室 |
| 作者单位 | 1.Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China 2.The Graduate School of the Chinese Academy of Sciences, Beijing 100009, China 3.Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, Chin |
| 推荐引用方式 GB/T 7714 | Chen ZM,Wu JB,Zhang Y,et al. Jerdonuxin, a novel snaclec (snake C-type lectin) with platelet aggregation activity from Trimeresurus jerdonii venom[J]. TOXICON,2011,57(1):109-116. |
| APA | Chen ZM.,Wu JB.,Zhang Y.,Yu GY.,Lee WH.,...&Zhang Y[*].(2011).Jerdonuxin, a novel snaclec (snake C-type lectin) with platelet aggregation activity from Trimeresurus jerdonii venom.TOXICON,57(1),109-116. |
| MLA | Chen ZM,et al."Jerdonuxin, a novel snaclec (snake C-type lectin) with platelet aggregation activity from Trimeresurus jerdonii venom".TOXICON 57.1(2011):109-116. |
入库方式： OAI收割
来源：昆明动物研究所
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