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A novel natriuretic peptide from the cobra venom
文献类型：期刊论文
| 作者 | Zhang Yong1; Wu JB1; Yu GY1,2; Chen ZM1; Zhou XD1; Zhu SW1; Li R1; Zhang Y1; Lu QM[*]1 |
| 刊名 | TOXICON
 |
| 出版日期 | 2011 |
| 卷号 | 57期号:1页码:134-140 |
| 关键词 | Natriuretic peptide Elapid snake Purification Molecular cloning |
| 通讯作者 | lvqm@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | Natriuretic peptides (NPs) play crucial roles in human physiology and pathophysiology through natriuresis, dieresis and vasorelaxation. NPs are also one of the important components of snake venoms. However, the low abundance in snake venom hampered the investigation. Here, a novel natriuretic peptide named Na-NP was purified from the cobra Naja atra venom. Na-NP consists of 45 amino acid residues and its molecular weight is 4618.5 Da. A full-length cDNA encoding Na-NP was obtained from the cDNA library constructed from the venom gland. The open reading frame of cloned Na-NP was composed of 498 bp and coded for a 165-amino acid residue protein precursor. The nucleotide and deduced protein sequences of Na-NP were remarkably conserved with other elapid NPs while significant different from the viperid NPs. Na-NP showed weak activity to relax the aortic rings pre-contracted with phenylephrine. Meanwhile, Na-NP showed cGMP-promotion activity against primary cultured rabbit endocardial endothelial cells, but had no effect on human platelet aggregation. In conclusion, this is the first report of a natriuretic peptide from the cobra N. atra venom. Na-NP might be served as a useful tool for the study of human NPs and the development of novel therapeutic drugs. (C) 2010 Elsevier Ltd. All rights reserved |
| 资助信息 | This work was supported by Western Light Project of Chinese Academy of Sciences, the Chinese National Natural Science Foundation (30770431), and Yunnan Natural Science Foundation (2007C102M). |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2011-03-21 |
| 源URL | [http://159.226.149.42:8088/handle/353002/6590]  |
| 专题 | 昆明动物研究所_动物毒素室 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 昆明动物研究所_动物活性蛋白多肽组学 昆明动物研究所_其他 |
| 作者单位 | 1.Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China 2.Kunming Med Coll, Dept Biochem, Kunming 650032, Yunnan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang Yong,Wu JB,Yu GY,et al. A novel natriuretic peptide from the cobra venom[J]. TOXICON,2011,57(1):134-140. |
| APA | Zhang Yong.,Wu JB.,Yu GY.,Chen ZM.,Zhou XD.,...&Lu QM[*].(2011).A novel natriuretic peptide from the cobra venom.TOXICON,57(1),134-140. |
| MLA | Zhang Yong,et al."A novel natriuretic peptide from the cobra venom".TOXICON 57.1(2011):134-140. |
入库方式： OAI收割
来源：昆明动物研究所
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