A novel platelet glycoprotein Ib-binding protein with human platelet aggregation-inhibiting activity from Trimeresurus jerdonii venom
文献类型：期刊论文
| 作者 | Chen ZM1,2; Wu JB3; Zhang Y1; Yang XW1,2; Yu GY1,2; Zhu SW3; Lee WH1; Zhang Y[*]1; Lu QM[*]3 |
| 刊名 | TOXICON
 |
| 出版日期 | 2011 |
| 卷号 | 57期号:5页码:672-679 |
| 关键词 | Jerdonibitin Purification Molecular cloning Glycoprotein Ib-binding Inhibition Platelet aggregation |
| 通讯作者 | lvqm@mail.kiz.ac.cn ; zhangy@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | Platelet glycoprotein Ib (GPIb) is a primary adhesion receptor and involved in platelet-related disorders. However, it is difficult to study GPIb-specific platelet stimulation using physiological ligands in vivo. GPIb-binding snake C-type lectins (snaclecs) are useful tools for exploring GPIb in vitro because they act on platelets differently. In the present study, a novel GPIb-binding snaclec, named jerdonibitin, was purified, molecular cloned and characterized from Trimeresurus jerdonii venom. On SDS-polyacrylamide gel electrophoresis, it showed a single band with an apparent molecular weight of 25 kDa under non-reducing conditions and two distinct bands with apparent molecular weights of 15 kDa (alpha-subunit) and 13 kDa (beta-subunit) under reducing conditions. The cDNA sequences of each subunit of jerdonibitin were identified and both deduced amino acid sequences were confirmed by N-terminal protein sequencing and trypsin-digested peptide mass fingerprinting of MALDI-TOF. Sequence alignment showed that jerdonibitin is a snaclec and has sequence similarity with TSV-GPIb-BP (a GPIb-inhibitory snaclec). Jerdonibitin dose-dependently inhibited platelet aggregation induced by ristocetin or low-dose thrombin, but not by high-dose thrombin. The GPlb alpha was detected by affinity chromatography on jerdonibitin. In vivo, jerdonibitin also dose-dependently induced thrombocytopenia of mice and platelet counts remained at very low level after 18 h intravenous injection. In summary, a novel GPIb-inhibitory snaclec was molecular cloned and characterized, which might provide insights into investigation of how GPIb-inhibitory snaclecs work and development of new antiplatelet agents |
| 资助信息 | This work was supported by grants from National Basic Research Program of China (973 Program, 2010CB529800), the Chinese National Natural Science Foundation (30630014, 30770431 and 30670412) and the Chinese Academy of Sciences “Key Research Direction” (KSCX2-YW- R-088, KSCX2-YW-R-212), the Western Light Project of CAS, Chinese National Natural Science Foundation (30770431), Yunnan Natural Science Foundation (2007C102M). |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2011-06-13 |
| 源URL | [http://159.226.149.42:8088/handle/353002/6619]  |
| 专题 | 昆明动物研究所_动物活性蛋白多肽组学 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 昆明动物研究所_动物毒素室 昆明动物研究所_其他 |
| 作者单位 | 1.Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming 650223, Yunnan, China 2.The Graduate School of the Chinese Academy of Sciences, Beijing 100009, China 3.Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming 650223, Yunnan, China |
| 推荐引用方式 GB/T 7714 | Chen ZM,Wu JB,Zhang Y,et al. A novel platelet glycoprotein Ib-binding protein with human platelet aggregation-inhibiting activity from Trimeresurus jerdonii venom[J]. TOXICON,2011,57(5):672-679. |
| APA | Chen ZM.,Wu JB.,Zhang Y.,Yang XW.,Yu GY.,...&Lu QM[*].(2011).A novel platelet glycoprotein Ib-binding protein with human platelet aggregation-inhibiting activity from Trimeresurus jerdonii venom.TOXICON,57(5),672-679. |
| MLA | Chen ZM,et al."A novel platelet glycoprotein Ib-binding protein with human platelet aggregation-inhibiting activity from Trimeresurus jerdonii venom".TOXICON 57.5(2011):672-679. |
入库方式： OAI收割
来源：昆明动物研究所
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