A Novel Bioactive Peptide with Myotropic Activity from Wasp Venoms
文献类型：期刊论文
| 作者 | Yan HL1; Chen WL2; Chen LL2; Lai R[*]3; Liu JZ4 |
| 刊名 | Chinese Journal of Natural Medicines
 |
| 出版日期 | 2011 |
| 卷号 | 9期号:4页码:317-320 |
| 关键词 | Wasp venoms Vespa bicolor Smooth muscle Contraction |
| 其他题名 | 黄蜂毒液中分离得到的一个有亲肌活性的毒素肽 |
| 通讯作者 | rlai@mail.kiz.ac.cn |
| 中文摘要 | 目的:研究从山西省采集的 Vespa bicolor Fabricius 毒液的化学组分。方法:用凝胶色谱和 HPLC 从 Vespa bicolor Fabricius 的毒液里分离多肽,并得到 1 个毒素肽。通过质谱和 Edman 降解法分析它的化学结构特征。从毒液腺体 cDNA 文库中克隆出编码它的前体的 cDNA。用合成肽做活性检测。结果:纯化检测出了 1 个具有独特的初级结构的新的毒素肽 (vespin-BF)。它的氨基酸序列测定为 TYQRKMAITAGAVKHRLMS TTIIIILVRIE YLRDNMVISLESSF。Vespin-BF 能够诱导离体回肠平滑肌收缩。它的前体由 67 个氨基酸残基组成,含有预测到的信号肽和成熟肽部分。在信号肽和成熟肽之间有个-KR-位点。BLAST 搜索到 vespin-BF 和从 Vespa magnifica 毒液中鉴定的 vespin 有明显的相似性。结论: 从黄蜂毒液中纯化出了 1 个新的毒素肽. |
| 英文摘要 | AIM: To study the chemical constituents of the venom of Vespa bicolor Fabricius collected from Shanxi Province, China. METHODS: Gel chromatography and HPLC were applied to isolate a peptide from the venom. Mass spectrometry and Edman degradation were used for its structural characterization. The cDNA encoding vespin-BF precursor was cloned from the cDNA library of the venomous glands. The synthetic peptide was used for its bioassay. RESULTS: A novel bioactive peptide (vespin-BF) with unique primary structure was purified and characterized. Its amino acid sequence was determined as TYQRKMAITAGAVKHRLMSTTIIIILVRIE YLRDNMVISLESSF. Vespin-BF induced contraction of isolated ileum smooth muscle. The precursor is composed of 67 amino acid residues including the predicted signal peptide and mature vespin-BF. A di-basic enzy- matic processing site (-KR-) was located between the signal and the mature peptide. BLAST search indicated that vespin-BF shows obvious similarity to vespin identified from the venoms of Vespa magnifica. CONCLUSION: A novel bioactive peptide from the wasp venoms was characterized. |
| 收录类别 | 其他 |
| 资助信息 | This project was supported by the Ministry of Agriculture (No. 2009ZX08009-159B). |
| 语种 | 中文 |
| 公开日期 | 2011-08-23 |
| 源URL | [http://159.226.149.42:8088/handle/353002/6677]  |
| 专题 | 昆明动物研究所_动物毒素室 |
| 作者单位 | 1.Life Sciences College of Nanjing Agricultural University, Nanjing 210095, China 2.Yunnan Clinical Research Center of Breast Cancer, the Third Affiliated Hospital of Kunming Medical College, Kunming 650032, China 3.Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 4.College of Life Sciences, Hebei Normal University, Shijiazhuang 050016, China |
| 推荐引用方式 GB/T 7714 | Yan HL,Chen WL,Chen LL,et al. A Novel Bioactive Peptide with Myotropic Activity from Wasp Venoms[J]. Chinese Journal of Natural Medicines,2011,9(4):317-320. |
| APA | Yan HL,Chen WL,Chen LL,Lai R[*],&Liu JZ.(2011).A Novel Bioactive Peptide with Myotropic Activity from Wasp Venoms.Chinese Journal of Natural Medicines,9(4),317-320. |
| MLA | Yan HL,et al."A Novel Bioactive Peptide with Myotropic Activity from Wasp Venoms".Chinese Journal of Natural Medicines 9.4(2011):317-320. |
入库方式： OAI收割
来源：昆明动物研究所
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