Two antimicrobial and nematicidal peptides derived from sequences encoded Picea sitchensis
文献类型：期刊论文
| 作者 | Liu R1; Mu LX2; Liu H2; Wei L1; Yan TH3; Chen M4; Zhang KY1; Li JX2; You DW2; Lai R[*]1,2 |
| 刊名 | JOURNAL OF PEPTIDE SCIENCE
 |
| 出版日期 | 2011 |
| 卷号 | 17期号:9页码:627-631 |
| 关键词 | Picea sitchensis antimicrobial and nematicidal peptides innate immunity |
| 通讯作者 | rlai72@njau.edu.cn |
| 合作状况 | 其它 |
| 英文摘要 | Two antimicrobial peptides (piceain 1 and 2) derived from sequences encoded Picea sitchensis are identified. Their amino acid sequences are KSLRPRCWIKIKFRCKSLKF and RPRCWIKIKFRCKSLKF, respectively. One intra-molecular disulfide bridge is formed by these two half-cysteines in both piceain 1 and 2. Antimicrobial activities of synthesized piceains against several kinds of microorganisms were tested. They showed antimicrobial activities against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and fungus Candida albicans but little antimicrobial activity against Bacillus subtilis. The results of nematicidal test showed they exerted strong nematicidal activities against Caenorhabditis elegans, following exposure for 5 h at concentrations as low as 10 mu g/ml. They had weak hemolytic abilities against human and rabbit red cells. At the concentration of 250 mu g/ml, they induced red cell hemolysis of less than 5%. Circular dichroism spectra of the two antimicrobial peptides were investigated in several solutions. Their main secondary structure components are beta-sheet and random. The current work provides a novel family of antimicrobial and nematicidal peptides with unique disulfided loop containing nine amino acid residues. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by Chinese National Natural Science Foundation (30830021,31000962,31070701,31025025),the Min- istry of Science and Technology (2010CB529800, 2009ZX09103- 1/091, 2011ZX09102-002-10) and the Ministry of Agriculture (2009ZX08009-159B). |
| 语种 | 英语 |
| 公开日期 | 2011-09-19 |
| 源URL | [http://159.226.149.42:8088/handle/353002/6784]  |
| 专题 | 昆明动物研究所_动物毒素室 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 |
| 作者单位 | 1.Laboratory of Microbiological Engineering of Agricultural Environment, MinistryofAgriculture,LifeSciencesCollegeofNanjingAgriculturalUniversity, Nanjing,Jiangsu,210095,Chin 2.Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming650223, Yunnan, China 3.DepartmentofPhysiology,ChinaPharmaceuticalUniversity,Nanjing,Jiangsu, 210095,China 4.The Department of Nephrology, Teaching Hospital of Chengdu University of TraditionalChineseMedical,Chengdu610072,China |
| 推荐引用方式 GB/T 7714 | Liu R,Mu LX,Liu H,et al. Two antimicrobial and nematicidal peptides derived from sequences encoded Picea sitchensis[J]. JOURNAL OF PEPTIDE SCIENCE,2011,17(9):627-631. |
| APA | Liu R.,Mu LX.,Liu H.,Wei L.,Yan TH.,...&Lai R[*].(2011).Two antimicrobial and nematicidal peptides derived from sequences encoded Picea sitchensis.JOURNAL OF PEPTIDE SCIENCE,17(9),627-631. |
| MLA | Liu R,et al."Two antimicrobial and nematicidal peptides derived from sequences encoded Picea sitchensis".JOURNAL OF PEPTIDE SCIENCE 17.9(2011):627-631. |
入库方式： OAI收割
来源：昆明动物研究所
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