A novel allergen Tab y 1 with inhibitory activity of platelet aggregation from salivary glands of horseflies
文献类型：期刊论文
| 作者 | An S1,3,4; Ma D1,4; Wei JF2; Yang X1,3; Yang HW2; Yang H1,4; Xu X1; He S[*]2; Lai R[*]1 |
| 刊名 | ALLERGY
 |
| 出版日期 | 2011 |
| 卷号 | 66期号:11页码:1420-1427 |
| 关键词 | apyrase basophil CD63 horsefly allergen insect allergy |
| 通讯作者 | shoahenghe@hotmail.com ; rlai@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | Background: Horsefly sting causes allergic reactions in human body. However, our knowledge on horsefly allergens remains poor. Objectives: To identify the novel horsefly allergens and characterize their properties. Methods: A native allergen protein Tab y 1 (apyrase) was purified from the salivary glands of the horsefly Tabanus yao Macquart by gel filtration and ion exchange chromatography. Its sequence was determined by Edman degradation and cDNA cloning. Its allergenicity was assessed by immunoblotting for specific IgE, basophil activation test, skin prick test (SPT), and competitive enzyme-linked immunosorbent assay (ELISA). Results: Tab y 1 showed a single diffusion band of 70 kDa on SDS-PAGE. Seventy percent (7/10) of patients with horsefly allergy tested positive to Tab y 1 in SPT; sera from 81% (30/37) of patients reacted to Tab y 1 on western blots. Purified Tab y 1 reduced approximately 42% sera IgE reactivity to horsefly salivary gland extract on a competitive ELISA. Tab y 1 upregulated the expression of CD63 and CCR3 on passively sensitized basophils by up to approximately 4.9-fold. Tab y 1 also showed enzymatic activity to hydrolyze ATP and ADP, and potent antiplatelet aggregation and antithrombotic activities. Conclusion: The current work identified a novel major allergen of horsefly, Tab y 1, with antiplatelet aggregation and antithrombotic activities, which implicates Tab y 1 in helping horseflies suck host blood, meanwhile causing allergy in their human hosts. |
| 收录类别 | SCI |
| 资助信息 | The work was supported by NSFC (30830021, 30800185, 30972822, 31025025), MOST (2010CB 529800, 2007CB512400, 2009ZX09103-1/091), and the Minis- try of Agriculture (2009ZX08009-159B). |
| 语种 | 英语 |
| 公开日期 | 2011-11-25 |
| 源URL | [http://159.226.149.42:8088/handle/353002/6826]  |
| 专题 | 昆明动物研究所_动物毒素室 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 |
| 作者单位 | 1.Biotoxin Units of Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 2.Clinical Research Centre, the First Affiliated Hospital of Nanjing Medical University, Nanjing 3.School of Life Sciences, University of Science and Technology of China, Hefe 4.Graduate School of the Chinese Academy of Sciences, Beijing, China |
| 推荐引用方式 GB/T 7714 | An S,Ma D,Wei JF,et al. A novel allergen Tab y 1 with inhibitory activity of platelet aggregation from salivary glands of horseflies[J]. ALLERGY,2011,66(11):1420-1427. |
| APA | An S.,Ma D.,Wei JF.,Yang X.,Yang HW.,...&Lai R[*].(2011).A novel allergen Tab y 1 with inhibitory activity of platelet aggregation from salivary glands of horseflies.ALLERGY,66(11),1420-1427. |
| MLA | An S,et al."A novel allergen Tab y 1 with inhibitory activity of platelet aggregation from salivary glands of horseflies".ALLERGY 66.11(2011):1420-1427. |
入库方式： OAI收割
来源：昆明动物研究所
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