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Amphibian cathelicidin fills the evolutionary gap of cathelicidin in vertebrate

文献类型：期刊论文

作者Hao X1,2; Yang HL1,3; Wei L1,2; Yang SL1,3; Zhu WJ4; Ma DY1; Yu HN[*]4,5; Lai R[*]1,2
刊名AMINO ACIDS
出版日期2012
卷号43期号:2页码:677-685
关键词Cathelicidin Amphibian Evolution Structure and function
通讯作者joannyu@live.cn ; rlai@mail.kiz.ac.cn
合作状况其它
英文摘要Cathelicidins comprise a family of antimicrobial peptides (AMPs) sharing a highly conserved cathelin domain, and play a central role in the innate defense against infection in most of vertebrates. But so far it has not yet been found in amphibians although a large number of other groups of AMPs have been identified. In the current work, the first amphibian cathelicidin (cathelicidin-AL) has been characterized from the frog skin of Amolops loloensis. Cathelicidin-AL (RRSRRGRGGGRRGGSGGRGGRGGGGRSGAGSSIAGVGSRGGGGGRHYA) is a cationic peptide containing 48 amino acid residues (aa) with 12 basic aa and no acidic aa. The chemical synthesized peptide efficiently killed bacteria and some fungal species including clinically isolated drug-resistance microorganisms. The cDNA encoding cathelicidin-AL precursor was cloned from the skin cDNA library of A. loloensis. As other cathelicidins, the precursor of cathelicidin-AL also contains highly conserved anionic cathelin domain of cysteine proteinase inhibitor followed by the AMP fragment at C-terminus. Phylogenetic analysis revealed that as connecting link, the amphibian cathelicidin predates reptilia but postdates fish cathelicidin. The peptide purification combined with gene cloning results confirms the presence of cathelicidin in amphibians and filled the evolutionary gap of cathelicidin in vertebrate, considering amphibians' special niche as the animals bridging the evolutionary land-water gap.
收录类别SCI
资助信息This work was funded by the grants from Chi- nese National Natural Science Foundation (30900240, 41076098).
语种英语
WOS记录号WOS:000306365500016
公开日期2012-09-04
源URL[http://159.226.149.42:8088/handle/152453/7009]  
专题昆明动物研究所_动物毒素室
昆明动物研究所_动物模型与人类重大疾病机理重点实验室
作者单位1.Biotoxin Units of Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China
2.Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing 210095, Jiangsu, China
3.Graduate School of the Chinese Academy of Sciences, Beijing 100009, China
4.School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, Liaoning, China
5.College of Life Sciences, Hebei Normal University, Shijiazhuang 050016, Hebei, China
推荐引用方式
GB/T 7714		 Hao X,Yang HL,Wei L,et al. Amphibian cathelicidin fills the evolutionary gap of cathelicidin in vertebrate[J]. AMINO ACIDS,2012,43(2):677-685.
APA Hao X.,Yang HL.,Wei L.,Yang SL.,Zhu WJ.,...&Lai R[*].(2012).Amphibian cathelicidin fills the evolutionary gap of cathelicidin in vertebrate.AMINO ACIDS,43(2),677-685.
MLA Hao X,et al."Amphibian cathelicidin fills the evolutionary gap of cathelicidin in vertebrate".AMINO ACIDS 43.2(2012):677-685.

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来源：昆明动物研究所

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