Snake venom-like waprin from the frog of Ceratophrys calcarata contains antimicrobial function
文献类型：期刊论文
| 作者 | Liu DX1; Wang YW1; Wei L1; Ye HH4; Liu H2; Wang L3; Liu R1; Li DS2; Lai R[*]1,2 |
| 刊名 | GENE
 |
| 出版日期 | 2013 |
| 卷号 | 514期号:2页码:99-104 |
| 关键词 | Amphibian Skin Venom Antimicrobial Innate immunity |
| 通讯作者 | rlai72@njau.edu.cn |
| 合作状况 | 其它 |
| 英文摘要 | A 255-bp cDNA encoding an 84-amino acid residue (aa) precursor protein containing 8 half-cysteines was cloned from the skin of the frog, Ceratophrys calcarata. By sequence comparison and signal peptide prediction, the precursor was predicted to release a 63-aa mature peptide with amino acid sequence, NVTPATKPTPSKPGYCRVMDELILCPDPPLSKDLCKNDSDCPGAQKCCYRTCIMQCLPPIFRE. The mature was named ceratoxin. Ceratoxin shares significant sequence similarity with the toxin family of waprins containing the whey acidic protein-type (WAP) four-disulfide core domain found in snake venoms. Antimicrobial and trypsin-inhibitory abilities of recombinant ceratoxin were tested. Recombinant ceratoxin showed strong antimicrobial activities against wide spectrum of microorganisms including Gram-negative and Gram-positive bacteria and fungi. It had no serine protease-inhibitory activity. The current results suggested that the snake venom-like waprin with antimicrobial activities in the frog skin plays a role in innate immunity. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by the Chinese National Natural Science Foundation (30830021, 31025025, 31070701, 31000960, 31025025, U1132601), the Ministry of Science and Technology (2010CB529800, 2009ZX09103-1/091, 2011ZX09102-002-10), and Yunnan Province Y103951111. |
| 语种 | 英语 |
| WOS记录号 | WOS:000315007600004 |
| 公开日期 | 2013-03-14 |
| 源URL | [http://159.226.149.42:8088/handle/152453/7276]  |
| 专题 | 昆明动物研究所_动物毒素室 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 |
| 作者单位 | 1.Life Sciences College of Nanjing Agricultural University, Nanjing 210095, Jiangsu, China 2.Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China 3.Food Science and Technology College of Guangdong Ocean University, Zhanjiang 524088, Guangdong, China 4.Laboratory Animal Center, Academy of Military Medical Sciences, Beijing, 100071, China |
| 推荐引用方式 GB/T 7714 | Liu DX,Wang YW,Wei L,et al. Snake venom-like waprin from the frog of Ceratophrys calcarata contains antimicrobial function[J]. GENE,2013,514(2):99-104. |
| APA | Liu DX.,Wang YW.,Wei L.,Ye HH.,Liu H.,...&Lai R[*].(2013).Snake venom-like waprin from the frog of Ceratophrys calcarata contains antimicrobial function.GENE,514(2),99-104. |
| MLA | Liu DX,et al."Snake venom-like waprin from the frog of Ceratophrys calcarata contains antimicrobial function".GENE 514.2(2013):99-104. |
入库方式： OAI收割
来源：昆明动物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。