Expression Patterns of the Cannabinoid Receptor 1 Gene in Pig Skeletal Muscle and Skeletal Satellite Muscle Cells
文献类型：期刊论文
| 作者 | Xu E1,2; Zhu LN1; Lai R3; Guo J1; Huang YN1; Wu T1; Wang YZ[*]1 |
| 刊名 | JOURNAL OF ANIMAL AND VETERINARY ADVANCES
 |
| 出版日期 | 2012 |
| 卷号 | 11期号:23页码:4297-4303 |
| 关键词 | Cannabinoid Receptor 1 (CNR1) skeletal muscle skeletal satellite muscle cells pig isolation gastrocnemius China |
| 合作状况 | 其它 |
| 英文摘要 | The Cannabinoid Receptor type 1 (CNR1) is a key component of the endocannabinoid system which has been reported to play a pivotal role in modulating feeding behavior and energy balance. While a large number of researches have showed clearly that CNR1 is expressed in skeletal muscle of mice and human beings, detailed analysis of CNR1 gene expression in pig muscles and muscle cells has not been reported. Here, quantitative PCR were used to characterize the CNR1 in different muscles of pig and isolation of porcine myogenic satellite cells. Results showed that the expression of CNR1 mRNA was greatest in longissimus dorsi muscle (p<0.01) among that in the gastrocnemius (mix), soleus muscle (slow-type) and longissimus doris muscle (fast-type). As to age-related changes of CNR1 gene in different pig muscles, the higher expression was found at the 30th day, then declined (p<0.05) in all three kinds of muscles to the 90th day. At the 150th day, the expression of CNR1 steadily decreased (p<0.05) in. the gastrocnemius compared to the 90th day but not in longissimus doris muscle and soleus. Meanwhile the breed differences in the expression of CNR1 mRNA between Jinhua pig and Landrace were also identified. The mRNA abundance of CNR1 in Landrace was higher compared with that of Jinhua pigs and strong differences were observed in longissimus doris muscle (p<0.05) and soleus (p<0.01). In vitro with the differentiation of the satellite cells, the mRNA expression of the CNR1 was significantly down-regulated (p<0.05). The lowest mRNA expression was observed at the 9th day. These data provide a framework of CNR1 gene expression patterns and implicate a novel gene for regulating muscle fiber types. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000317820600002 |
| 公开日期 | 2013-05-23 |
| 源URL | [http://159.226.149.42:8088/handle/152453/7441]  |
| 专题 | 昆明动物研究所_动物毒素室 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 |
| 作者单位 | 1.Zhejiang Univ, Zhejiang Prov Lab Feed & Anim Nutr, Key Lab Mol Anim Nutr,Minist Educ,Inst Feed Sci, Key Lab Anim Nutr & Feed Sci East China,Minist Ag, Hangzhou 310058, Zhejiang, Peoples R China 2.Guizhou Univ, Coll Anim Sci, Guiyang 550025, Peoples R China 3.Chinese Acad Sci, Kunming Inst Zool, Key Lab Anim Models & Human Dis Mech, Biotoxin Units, Kunming 650223, Yunnan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu E,Zhu LN,Lai R,et al. Expression Patterns of the Cannabinoid Receptor 1 Gene in Pig Skeletal Muscle and Skeletal Satellite Muscle Cells[J]. JOURNAL OF ANIMAL AND VETERINARY ADVANCES,2012,11(23):4297-4303. |
| APA | Xu E.,Zhu LN.,Lai R.,Guo J.,Huang YN.,...&Wang YZ[*].(2012).Expression Patterns of the Cannabinoid Receptor 1 Gene in Pig Skeletal Muscle and Skeletal Satellite Muscle Cells.JOURNAL OF ANIMAL AND VETERINARY ADVANCES,11(23),4297-4303. |
| MLA | Xu E,et al."Expression Patterns of the Cannabinoid Receptor 1 Gene in Pig Skeletal Muscle and Skeletal Satellite Muscle Cells".JOURNAL OF ANIMAL AND VETERINARY ADVANCES 11.23(2012):4297-4303. |
入库方式： OAI收割
来源：昆明动物研究所
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