Effects of Amino Acid Deletion and Substitution on the Chemical Properties, Biological Activities of the Frog Peptide Palustrin-OG1
文献类型：期刊论文
| 作者 | Xie YG1; Liu YF1; Luan C1; Han FF1; Lai R2; Groleau D3; Feng J1; Wang YZ[*]1 |
| 刊名 | PROTEIN AND PEPTIDE LETTERS
 |
| 出版日期 | 2013 |
| 卷号 | 20期号:7页码:813-819 |
| 关键词 | Palustrin-OG1 host defense peptide antimicrobial activity killing kinetics cytotoxicity |
| 通讯作者 | yzwang@zju.edu.cn |
| 合作状况 | 其它 |
| 英文摘要 | Palustrin-OG1 (OG1) is a host defense peptide isolated from the frog Odorrana grahami. In this study, we analyzed the chemical properties, antimicrobial activities and cytotoxicities of OG1 and its derivatives to identify the most promising peptide as an antimicrobial agent. By increasing the net positive charge, amphipathicity and decreasing the mean hydrophobicity of OG1, the derivative named as OG2 exerted higher antimicrobial activity against bacteria but lower cytotoxicity against both porcine erythrocytes and peripheral blood mononuclear cells than did OG1 (P<0.01). After substitution of Cys residues of OG2 by Ala or Trp residues, two derivatives named as OG2A and OG2W were less effective against bacteria and induced greater hemolysis than did OG2, indicating the importance of Cys residues. The substitution of the C-terminal Thr of OG2 resulted OG2N, which decreased the cytotoxicity and improved killing kinetics against gram-positive bacteria by the rapid damage of cell wall and |
| 收录类别 | SCI |
| 资助信息 | This study was supported by The National High Tech- nology Research and Development Program of China (863 Program, No. 2007AA100602), National 12th 5-year Plan (No. NC2010CA0026) and Foundation for the Author of National Excellent Doctoral Dissertation of China (FANEDD, Grant No. 2007B6). |
| 语种 | 英语 |
| WOS记录号 | WOS:000319499400012 |
| 公开日期 | 2013-07-05 |
| 源URL | [http://159.226.149.42:8088/handle/152453/7558]  |
| 专题 | 昆明动物研究所_动物毒素室 |
| 作者单位 | 1.Key laboratory of Animal Nutrition and Feed Science of Ministry of Agriculture, Key Laboratory of Feed and Animal Nutrition of Zhejiang Province, Institute of Feed Science, Zhejiang University, Hangzhou, Zhejiang, PR China 2.Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, PR China 3.Biotechnology Research Insti- tute, National Research Council, Montreal, Quebec, Canada |
| 推荐引用方式 GB/T 7714 | Xie YG,Liu YF,Luan C,et al. Effects of Amino Acid Deletion and Substitution on the Chemical Properties, Biological Activities of the Frog Peptide Palustrin-OG1[J]. PROTEIN AND PEPTIDE LETTERS,2013,20(7):813-819. |
| APA | Xie YG.,Liu YF.,Luan C.,Han FF.,Lai R.,...&Wang YZ[*].(2013).Effects of Amino Acid Deletion and Substitution on the Chemical Properties, Biological Activities of the Frog Peptide Palustrin-OG1.PROTEIN AND PEPTIDE LETTERS,20(7),813-819. |
| MLA | Xie YG,et al."Effects of Amino Acid Deletion and Substitution on the Chemical Properties, Biological Activities of the Frog Peptide Palustrin-OG1".PROTEIN AND PEPTIDE LETTERS 20.7(2013):813-819. |
入库方式： OAI收割
来源：昆明动物研究所
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