Dermatophagoides farinae allergens diversity identification by proteomics
文献类型：期刊论文
| 作者 | An S1,2,5,6; Chen LL4; Long CB1,2; Liu XY3; Xu XM4; Lu XR7; Rong MQ[*]1,2; Liu ZG[*]3; Lai R[*]1,2 |
| 刊名 | MOLECULAR & CELLULAR PROTEOMICS
 |
| 出版日期 | 2013 |
| 卷号 | 12期号:7页码:1818-1828 |
| 关键词 | Dermatophagoides farinae Allergen Proteomics Mite |
| 通讯作者 | rongmingqiang@mail.kiz.ac.cn ; rlai@mail.kiz.ac.cn ; ,lzg195910@126.com |
| 合作状况 | 其它 |
| 英文摘要 | The most important indoor allergens for humans are house dust mites (HDM). Fourteen Dermatophagoides farinae allergens (Der f 1-3, 6, 7, 10, 11, 13-18, and 22) are reported although more than 30 allergens have been estimated in D. farinae. Seventeen allergens belonging to 12 different groups were identified by a procedure of proteomics combined with two-dimensional immunoblotting from D. farina extracts. Their sequences were determined by Edman degradation, mass spectrometry analysis, and cDNA cloning. Their allergenicities were assayed by enzyme-linked immunosorbent assay inhibition tests, immunoblots, basophil activation test, and skin prick tests. Eight of them are the first report as D. farinae allergens. The procedure of using a proteomic approach combined with a purely discovery approach using sera of patients with broad IgE reactivity profiles to mite allergens was an effective method to investigate a more complete repertoire of D. farinae allergens. The identification of eight new D. farinae allergens will be helpful for HDM allergy diagnosis and therapy, especially for patients without response for HDM major allergens. In addition, the current work significantly extendedthe repertoire of D. farinae allergens. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by Chinese National Natural Science Foundation (31070701, 31000962, 31025025, 31200590, 30971179, 30972848, 31028006), the Ministry of Science and Technology (2010CB529800, 2009ZX09103-1/091, 011ZX09102-002-10, 2013CB911300). |
| 语种 | 英语 |
| 公开日期 | 2013-09-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/7677]  |
| 专题 | 昆明动物研究所_动物毒素室 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 |
| 作者单位 | 1.Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Science 2.Yunnan Province, Kunming Institute of Zoology, Kunming 650223, Yunnan, China 3.Institute of Allergy and Immunology, Shenzhen University, Shen- zhen, China 4.Clinical Laboratory, the First Affiliated Hospital of Kun- ming Medical College, Kunming 650032, Yunnan, China 5.School of Life Sciences, University of Science and Technology of China, Hefei 230026, Anhui, China 6.Clinical laboratory of Wenshan- zhou People Hospital, Wenshanzhou 663000, Yunnan, China 7.Clinical laboratory of Wenshan- zhou People Hospital, Wenshanzhou 663000, Yunnan, China |
| 推荐引用方式 GB/T 7714 | An S,Chen LL,Long CB,et al. Dermatophagoides farinae allergens diversity identification by proteomics[J]. MOLECULAR & CELLULAR PROTEOMICS,2013,12(7):1818-1828. |
| APA | An S.,Chen LL.,Long CB.,Liu XY.,Xu XM.,...&Lai R[*].(2013).Dermatophagoides farinae allergens diversity identification by proteomics.MOLECULAR & CELLULAR PROTEOMICS,12(7),1818-1828. |
| MLA | An S,et al."Dermatophagoides farinae allergens diversity identification by proteomics".MOLECULAR & CELLULAR PROTEOMICS 12.7(2013):1818-1828. |
入库方式： OAI收割
来源：昆明动物研究所
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