Electrophysiological Characterisation of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells Induced by Olfactory Ensheathing Cell-Conditioned Medium
文献类型：期刊论文
| 作者 | Zeng Y1; Rong MQ2; Liu YS1; Liu JF1; Lu M[*]3; Tao XY3; Li ZY1; Chen X1; Yang K1; Li CT1 |
| 刊名 | NEUROCHEMICAL RESEARCH
 |
| 出版日期 | 2013 |
| 卷号 | 38期号:12页码:2483-2489 |
| 关键词 | Stem cell Differentiation Olfactory ensheathing cells Ion channel |
| 通讯作者 | lming163@yahoo.com.cn ; zhixiongliu@csu.edu.cn |
| 合作状况 | 其它 |
| 英文摘要 | Umbilical cord blood-derived marrow stromal cells (UCB-MSCs) with high proliferation capacity and immunomodulatory properties are considered to be a good candidate for cell-based therapies. But until now, little work has been focused on the differentiation of UCB-MSCs. In this work, UCB-MSCs were demonstrated to be negative for CD34 and CD45 expression but positive for CD90 and CD105 expression. The gate values of UCB-MSCs for CD90 and CD105 were 99.3 and 98.6 %, respectively. Two weeks after treatment, the percentage of neuron-like cells differentiated from UCB-MSCs was increased to 84 +/- A 12 % in the experimental group [treated with olfactory ensheathing cells (OECs)-conditioned medium] and they were neuron-specific enolase positive; few neuron-like cells were found in the control group (without OECs-conditioned medium). Using whole-cell recording, sodium and potassium currents were recorded in UCB-MSCs after differentiation by OECs. Thus, human UCB-MSCs could be differentiated to neural cells by secreted secretion from OECs and exhibited electrophysiological properties similar to mature neurons after 2 weeks post-induction. These results imply that OECs can be used as a new strategy for stem cell differentiation and provide an alternative neurogenesis pathway for generating sufficient numbers of neural cells for cell therapy. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000327131500004 |
| 公开日期 | 2013-12-13 |
| 源URL | [http://159.226.149.42:8088/handle/152453/7726]  |
| 专题 | 昆明动物研究所_动物毒素室 |
| 作者单位 | 1.Department of Neurosurgery, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha, Hunan Province 410008, China 2.Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 3.Department of Neurosurgery, Second Affiliated Hospital of Hunan Normal University, 163 |
| 推荐引用方式 GB/T 7714 | Zeng Y,Rong MQ,Liu YS,et al. Electrophysiological Characterisation of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells Induced by Olfactory Ensheathing Cell-Conditioned Medium[J]. NEUROCHEMICAL RESEARCH,2013,38(12):2483-2489. |
| APA | Zeng Y.,Rong MQ.,Liu YS.,Liu JF.,Lu M[*].,...&Liu ZX[*].(2013).Electrophysiological Characterisation of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells Induced by Olfactory Ensheathing Cell-Conditioned Medium.NEUROCHEMICAL RESEARCH,38(12),2483-2489. |
| MLA | Zeng Y,et al."Electrophysiological Characterisation of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells Induced by Olfactory Ensheathing Cell-Conditioned Medium".NEUROCHEMICAL RESEARCH 38.12(2013):2483-2489. |
入库方式： OAI收割
来源：昆明动物研究所
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