Two new types of allergens from the cockroach, Periplaneta americana
文献类型：期刊论文
| 作者 | Fang Y1; Long C1; Bai X1; Liu W1; Rong M1; Lai R[*]1,2,3; An S[*]1,4 |
| 刊名 | ALLERGY
 |
| 出版日期 | 2015 |
| 卷号 | 70期号:12页码:1674-1678 |
| 关键词 | alpha-amylase anaphylaxis basophil chitinase cockroach allergens |
| 通讯作者 | rlai@mail.kiz.ac.cn ; aslxj@mail.ustc.edu.cn |
| 合作状况 | 其它 |
| 英文摘要 | Periplaneta americana cockroach is an important source of inhalant indoor allergen resource, there are more than twenty IgE-binding components identified in P. americana, but only 9 allergens were characterized. Our knowledge about cockroach allergens remains poor. In this work, two novel allergen proteins Per a 11 (alpha-amylase) and Per a 12 (chitinase) with molecular weight around 55 and 45 kDa, respectively, were purified and characterized from the midgut of cockroaches. Their primary sequences were determined by Edman degradation, mass spectrometry and cDNA cloning. Sera from 39 and 30 of 47 (83.0% and 63.8%) patients reacted to Per a 11 and Per a 12 on immunoblots, respectively. The allergenicity of Per a 11 and Per a 12 was further confirmed by competitive ELISA, basophil activation test (BAT) and skin prick test (SPT). They appear to be of importance for the allergic reactions induced by cockroach, and have a potential for component-based diagnosis of allergy. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by MOST (2013CB911300, 2010CB529800), NSFC (31025025, U1132601, 31200590, 81460253), CAS (SAJC201308), and Yunnan Pro- vince (2011CI139, 2012BC009). |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/9436]  |
| 专题 | 昆明动物研究所_动物毒素室 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 |
| 作者单位 | 1.Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming, Yunnan 2.Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu 3.Joint Laboratory of Natural peptide, Chinese Academy of Sciences, University of Science and Technology of China and Kunming Institute of Zoology 4.Faculty of Life Science and Technology, Kunming University of Science and Technology, Yunnan, China |
| 推荐引用方式 GB/T 7714 | Fang Y,Long C,Bai X,et al. Two new types of allergens from the cockroach, Periplaneta americana[J]. ALLERGY,2015,70(12):1674-1678. |
| APA | Fang Y.,Long C.,Bai X.,Liu W.,Rong M.,...&An S[*].(2015).Two new types of allergens from the cockroach, Periplaneta americana.ALLERGY,70(12),1674-1678. |
| MLA | Fang Y,et al."Two new types of allergens from the cockroach, Periplaneta americana".ALLERGY 70.12(2015):1674-1678. |
入库方式： OAI收割
来源：昆明动物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。