A novel ranacyclin-like peptide with anti-platelet activity identified from skin secretions of the frog Amolops loloensis
文献类型：期刊论文
| 作者 | Hao X1,2; Tang XP1,2; Lu QM[*]1,4; Luo L1,2; Wang YP3; Lai R1,4 |
| 刊名 | GENE
 |
| 出版日期 | 2016 |
| 卷号 | 576期号:1页码:171–175 |
| 关键词 | Amolops loloensis Amphibian Anti-platelet Skin secretion |
| 通讯作者 | lvqm@mail.kiz.ac.cn |
| 英文摘要 | Albeit many bioactive peptides have been reported from amphibian skins, no anti-platelet peptide has been identified till to date. Here, an anti-platelet peptide, namely Zongdian platelet inhibitor (ZDPI), with the molecular weight of 1798.6 Da, was purified and characterized from skin secretions of the frog, Amolops loloensis. The amino acid sequence of ZDPI was determined as FRGCWLKNYSPRGCL-NH2 by combination methods of Edman degradation, mass spectrometry analysis and carboxypeptidase Y treatment revealing that it is composed of 15 amino acid residues with two cysteines formed an intra-molecular disulfide bridge and C-terminal amidation. cDNA encoding ZDPI precursor was cloned from skin cDNA library of A. loloensis. The precursor is composed of 63 amino acid (aa) residues including the predicted signal peptide (22 aa), an acidic spacer peptide (19 aa), and mature ZDPI. BLAST search indicates that ZDPI belongs to antimicrobial peptide family of ranacyclin, peptide leucine arginine or odorranain. It was found to inhibit ADP-induced platelet aggregation in a dose-dependent manner. At the concentration of 32 μg/ml, ZDPI completely inhibited platelet aggregation induced by ADP. To the best of our knowledge, this is the first report about an anti-platelet peptide from amphibian skin secretions. Considering its strong inhibitory ability on platelets and simple structure, ZDPI might be an excellent candidate or template to develop anti-thrombosis agent. In addition, the discovery of anti-platelet peptide in the frog skin increases biological function spectrum of amphibian skin peptides. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by MOST (2013CB911300), NSFC (U1132601, 31200590), CAS (SAJC201308) and Yunnan Province (2011CI139, 2012BC009). |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/9442]  |
| 专题 | 昆明动物研究所_动物毒素室 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 |
| 作者单位 | 1.Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming 650223, Yunnan, China 2.Graduate University of Chinese Academy of Sciences, Beijing 100009, China 3.Clinical Laboratory, The Second Affiliated Hospital of Kunming Medical University, China 4.Joint Laboratory of Natural Peptide of University of Science and Technology of China & Kunming Institute of Zoology, Chinese Academy of Sciences, China |
| 推荐引用方式 GB/T 7714 | Hao X,Tang XP,Lu QM[*],et al. A novel ranacyclin-like peptide with anti-platelet activity identified from skin secretions of the frog Amolops loloensis[J]. GENE,2016,576(1):171–175. |
| APA | Hao X,Tang XP,Lu QM[*],Luo L,Wang YP,&Lai R.(2016).A novel ranacyclin-like peptide with anti-platelet activity identified from skin secretions of the frog Amolops loloensis.GENE,576(1),171–175. |
| MLA | Hao X,et al."A novel ranacyclin-like peptide with anti-platelet activity identified from skin secretions of the frog Amolops loloensis".GENE 576.1(2016):171–175. |
入库方式： OAI收割
来源：昆明动物研究所
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