Two novel Bv8-like peptides from skin secretions of the toad Bombina maxima
文献类型：期刊论文
| 作者 | Lai R; Liu H; Lee WH; Zhang Y[*] |
| 刊名 | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY Part B
 |
| 出版日期 | 2003 |
| 卷号 | 134期号:3页码:509-514 |
| 关键词 | Amphibian Bombina maxima Toad Bv8-like peptide Neurotrophic factor Vascular contraction cDNA Sequence |
| 通讯作者 | zhangy@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | Two novel bioactive peptides were purified from skin secretions of the toad Bombina maxima. The partial N-terminal sequences of these two peptides were determined by automated Edman degradation. This allowed the cloning of full-length cDNAs encoding these two peptides from a cDNA library prepared from the toad skin. The deduced complete amino acid sequences indicate that both peptides are composed of 77 amino acids. A FASTA search in the databanks revealed that they exhibit 86-91% sequence identity with Bv8, a peptide originally isolated from skin secretions of Bombina variegata. They were thus named as Bv8-like peptide 1 (Bv8-LP1) and Bv8-like peptide 2 (Bv8-LP2), respectively. Sequence differences between Bv8-LP1 and 2 were due to six amino acid substitutions at positions 6, 11, 23, 24, 62 and 63. Bv8-LP1 and 2 differed from Bv8 with eleven and seven amino acid substitutions, respectively. Like Bv8, Bv8-LP1 and 2 possessed contractile activity on isolated guinea pig ileum. Additionally, they stimulated contraction of rabbit aortic rings in a dose-dependent manner at nanomolar concentrations. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by the grants of ‘STZ98-3-01’, ‘Western Light Project’, and ‘Tenth Five Plan’ pre- research project from the Chinese Academy of Sciences; and by grants from the National Natural Science Foundation (30170195) and the Yunnan Science and Technology Commission (2001C0061M) to Dr Yun Zhang. |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/9674]  |
| 专题 | 昆明动物研究所_动物毒素室 昆明动物研究所_动物活性蛋白多肽组学 |
| 作者单位 | Department of Animal Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, 32 East Jiao Chang Road, Kunming, Yunnan 650223, PR China |
| 推荐引用方式 GB/T 7714 | Lai R,Liu H,Lee WH,et al. Two novel Bv8-like peptides from skin secretions of the toad Bombina maxima[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY Part B,2003,134(3):509-514. |
| APA | Lai R,Liu H,Lee WH,&Zhang Y[*].(2003).Two novel Bv8-like peptides from skin secretions of the toad Bombina maxima.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY Part B,134(3),509-514. |
| MLA | Lai R,et al."Two novel Bv8-like peptides from skin secretions of the toad Bombina maxima".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY Part B 134.3(2003):509-514. |
入库方式： OAI收割
来源：昆明动物研究所
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