A novel myotoxin from the venom of Trimeresurus mucrosquamatus
文献类型：期刊论文
| 作者 | Xu TR; Wang WY; Meng QX; Huang YH; Lu QM; Xiong YL[*] |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 1999 |
| 卷号 | 31期号:5页码:483-488 |
| 关键词 | myotoxin phospholipase A(2) platelet Trimeresurus mucrosquamatus venom |
| 其他题名 | 一种新的烙铁头蛇毒肌肉毒素 |
| 通讯作者 | xutr@163.net |
| 合作状况 | 其它 |
| 中文摘要 | 用分子筛和快速蛋白质液相色谱(FPLC)从烙铁头(Trimeresurus mucrosquamatus)蛇毒中分离了一个新的碱性肌肉 毒素, 命名为 TMPB。 它的分子量为 16 000, 等电点为 9.2。 用蛋白质序列仪测定了其 N 端 24 个氨基酸残基, TMPB 与其他两个从同种蛇毒中分离到的碱性磷酯酶 A 2 的同源性分别为 41.7%和 54.2%。 TMPB 没有明显的磷酯酶 A 2 水解活性, 但它的肌肉毒性和血小板聚集抑制活性却极强, 其肌肉毒性和血小板聚集抑制活性可被肝素所抑制。 |
| 英文摘要 | A novel myotoxin , designated TMPB , was purified from the venom of Trimeresurus mucrosquamatus by Sephadex G-100 superfine gel chromatography and fast protein liquid chromatography (FPLC).The N-terminal sequence of 24 amino acid residues was determined by protein sequencer.The sequence similarities between TMPB and other two phospholipase A 2 (PLA 2 s)previously purified from the same venom were 41.7 %and 54 .2%, respectively , but TMPB showed no detectable PLA 2 hydrolytic activity .Its molecular weight was estimated to be 16 000 by reducing SDS-PAGE and isoelectric point wasdetermined to be 9.2 by isoelectric focusing electrophoresis.TMPB exhibited strong myotoxicity and platelet aggregation inhibiting activity , and the two activities could all be inhibited by heparin. |
| 收录类别 | 其他 |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/9679]  |
| 专题 | 昆明动物研究所_动物毒素室 昆明动物研究所_其他 |
| 作者单位 | Kunming Institute of Zoology , the Chinese Academy of Sciences, K unming 650223, China |
| 推荐引用方式 GB/T 7714 | Xu TR,Wang WY,Meng QX,et al. A novel myotoxin from the venom of Trimeresurus mucrosquamatus[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,1999,31(5):483-488. |
| APA | Xu TR,Wang WY,Meng QX,Huang YH,Lu QM,&Xiong YL[*].(1999).A novel myotoxin from the venom of Trimeresurus mucrosquamatus.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,31(5),483-488. |
| MLA | Xu TR,et al."A novel myotoxin from the venom of Trimeresurus mucrosquamatus".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 31.5(1999):483-488. |
入库方式： OAI收割
来源：昆明动物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。