Purification and Characterization of a Novel Kazal-Type Trypsin Inhibitor from the Leech of Hirudinaria manillensis
文献类型：期刊论文
| 作者 | Lai YM1; Li BW2; Ombati R4,5; Lai R[*]2,4; Long CB[*]2; Li HY[*]1; Liu WH2; Wang G2; Du CW3 |
| 刊名 | TOXINS
 |
| 出版日期 | 2016 |
| 卷号 | 8期号:8页码:e229 |
| 关键词 | Hirudinaria manillensis Kazal-type trypsin inhibitor bdellin protease inhibitor |
| 通讯作者 | rlai@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | Kazal-type serine proteinase inhibitors are found in a large number of living organisms and play crucial roles in various biological and physiological processes. Although some Kazal-type serine protease inhibitors have been identified in leeches, none has been reported from Hirudinaria manillensis, which is a medically important leech. In this study, a novel Kazal-type trypsin inhibitor was isolated from leech H. manillensis, purified and named as bdellin-HM based on the sequence similarity with bdellin-KL and bdellin B-3. Structural analysis revealed that bdellin-HM was a 17,432.8 Da protein and comprised of 149 amino acid residues with six cysteines forming three intra-molecular disulfide bonds. Bdellin-HM showed similarity with the Kazal-type domain and may belong to the group of "non-classical" Kazal inhibitors according to its Cys(I)-Cys(II) disulfide bridge position. Bdellin-HM had no inhibitory effect on elastase, chymotrypsin, kallikrein, Factor (F) XIIa, FXIa, FXa, thrombin and plasmin, but it showed a potent ability to inhibit trypsin with an inhibition constant (Ki) of (8.12 ± 0.18) × 10(-9) M. These results suggest that bdellin-HM from the leech of H. manillensis plays a potent and specific inhibitory role towards trypsin. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by Chinese Academy of Sciences (Y302B01021), Ministry of Science and Technology ofChina (2013CB911300), NationalScience FoundationofChina(U1132601and 31200590), Yunnan Province (2015BC005 and P0120150010) and Sino-African Joint Research Center (SAJC201308 and SAJC201606). |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/10347]  |
| 专题 | 昆明动物研究所_动物毒素室 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 |
| 作者单位 | 1.Department of Endocrine and breast Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China 2.Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming 650223, China 3.Life Sciences College, Nanjing Agricultural University, Nanjing 210095, China 4.Sino-Africa Joint Research Center, Chinese Academy of Sciences, Kunming Institute of Zoology, Kunming 650223, Yunnan, China 5.Institute of Primate Research, P.O Box 24481, Nairobi, Kenya |
| 推荐引用方式 GB/T 7714 | Lai YM,Li BW,Ombati R,et al. Purification and Characterization of a Novel Kazal-Type Trypsin Inhibitor from the Leech of Hirudinaria manillensis[J]. TOXINS,2016,8(8):e229. |
| APA | Lai YM.,Li BW.,Ombati R.,Lai R[*].,Long CB[*].,...&Du CW.(2016).Purification and Characterization of a Novel Kazal-Type Trypsin Inhibitor from the Leech of Hirudinaria manillensis.TOXINS,8(8),e229. |
| MLA | Lai YM,et al."Purification and Characterization of a Novel Kazal-Type Trypsin Inhibitor from the Leech of Hirudinaria manillensis".TOXINS 8.8(2016):e229. |
入库方式： OAI收割
来源：昆明动物研究所
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