A novel bradykinin-like peptide from skin secretion of rufous-spotted torrent frog, Amolops lolensis
文献类型：期刊论文
| 作者 | Liang JG1; Han YP1,4; Lai R[*]1,2; Li JX2; Xu XQ2; Rees HH3 |
| 刊名 | PEPTIDES
 |
| 出版日期 | 2006 |
| 卷号 | 27期号:11页码:2683-2687 |
| 关键词 | amphibian bradykinin-peptide amolopkinin Amolops loloensis skin |
| 通讯作者 | rlai72@njau.edu.cn |
| 合作状况 | 其它 |
| 英文摘要 | A bradykinin-like peptide has been isolated from skin secretions of rufous-spotted torrent frog, Amolops loloensis. This bradykinin-like peptide was named amolopkinin. Its primary structure, RAPVPPGFTPFR, was determined by Edman degradation and mass spectrometry. It is structurally related to bradykinin-like peptides identified from skin secretions of other amphibians. Amolopkinin is composed of 12 amino acid residues and is related to bradykinin composed of nine amino acid residues, identified from the skin secretions of Odorrana schmackeri. Amolopkinin was found to elicit concentration-dependent contractile effects on isolated guinea pig ileum. cDNA clones encoding the precursor of amolopkinin were isolated by screening a skin cDNA library of A. loloensis and then sequenced. The amino acid sequences deduced from the cDNA sequences match well with the results from Edman degradation. Analysis of different amphibian bradykinin cDNA structures revealed that a deficiency of an18-nucleotide fragment (TCAAGAATGATCAGACGC in the cDNA encoding bradykinin from O. schmackeri) in the peptide-coding region resulted in absence of a di-basic site for trypsin-like proteinases and an unusual - APV - insertion in the N-terminal part of amolopkinin. This is the first report of a bradykinin-like peptide comprised of bradykinin with an insertion in its N-terminal part. Our results demonstrate the hypervariability of amphibian bradykinin-like peptides, as well as the diversity of antimicrobial peptides in amphibians. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by the Chinese National Natural Science Foundation (30200044, 30570360), Yunnan Natural Science Foundation (2005C0054M) andJiangsu Natural Science Foundation (BK2005422). |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/10551]  |
| 专题 | 昆明动物研究所_动物毒素室 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 |
| 作者单位 | 1.Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu 210095, China 2.Biotoxin Department of Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650223 Yunnan, China 3.School of Biological Sciences, University of Liverpool, Liverpool L69 7ZB, United Kingdom 4.Department of Life Science and Technology, Changshu Institute of Technology, Changshu, Jiangsu 215500, China |
| 推荐引用方式 GB/T 7714 | Liang JG,Han YP,Lai R[*],et al. A novel bradykinin-like peptide from skin secretion of rufous-spotted torrent frog, Amolops lolensis[J]. PEPTIDES,2006,27(11):2683-2687. |
| APA | Liang JG,Han YP,Lai R[*],Li JX,Xu XQ,&Rees HH.(2006).A novel bradykinin-like peptide from skin secretion of rufous-spotted torrent frog, Amolops lolensis.PEPTIDES,27(11),2683-2687. |
| MLA | Liang JG,et al."A novel bradykinin-like peptide from skin secretion of rufous-spotted torrent frog, Amolops lolensis".PEPTIDES 27.11(2006):2683-2687. |
入库方式： OAI收割
来源：昆明动物研究所
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