Variety of antimicrobial peptides in the Bombina maxima toad and evidence of their rapid diversification
文献类型：期刊论文
| 作者 | Lee WH1; Li Y2,3; Wang W[*]2; Lai R1; Li S2,4; Zhang Y[*]1 |
| 刊名 | EUROPEAN JOURNAL OF IMMUNOLOGY
 |
| 出版日期 | 2005 |
| 卷号 | 35期号:4页码:1220-1229 |
| 关键词 | antimicrobial peptides rapid diversification Bombina maxima |
| 通讯作者 | zhangy@mail.kiz.ac.cn ; wwang@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | Antimicrobial peptides secreted by the skin of many amphibians play an important role in innate immunity. From two skin cDNA libraries of two individuals of the Chinese red belly toad (Bombina maxima), we identified 56 different antimicrobial peptide cDNA sequences, each of which encodes a precursor peptide that can give rise to two kinds of antimicrobial peptides, maximin and maximin H. Among these cDNA, we found that the mean number of nucleotide substitution per non-synonymous site in both the maximin and maximin H domains significantly exceed the mean number of nucleotide substitution per synonymous site, whereas the same pattern was not observed in other structural regions, such as the signal and propiece peptide regions, suggesting that these antimicrobial peptide genes have been experiencing rapid diversification driven by Darwinian selection. We cloned and sequenced seven genes amplified from skin or liver genomic DNA. These genes have three exons and share the same gene structure, in which both maximin and maximin H are encoded by the third exon. This suggests that alternative splicing and somatic recombination are less likely to play a role in creating the diversity of maximins and maximin Hs. The gene trees based on different domain regions revealed that domain shuffling or gene conversion among these genes might have happened frequently. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by “Western Light” projects from CAS and grants from NSFC (30170195, 30470380) and NSF of Yunnan Province (2001C0061M, 2003C0066M) to Y. Zhang and W.-H. Lee, and a CAS-Max Planck Society Fellowship, a CAS key project grant (No. KSCX2-SW-121), and an NSFC award for young scientists (No. 30325016) to W. Wang. |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/10607]  |
| 专题 | 昆明动物研究所_动物毒素室 昆明动物研究所_动物活性蛋白多肽组学 昆明动物研究所_基因起源组 昆明动物研究所_细胞与分子进化重点实验室 |
| 作者单位 | 1.Department of Animal Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences (CAS), Kunming, P. R. China 2.CAS-Max Planck Junior Scientist Group, Key Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, The Chinese Academy of Sciences (CAS), Kunming, P. R. China 3.Graduate School of the Chinese Academy of Sciences, Beijing, P. R. China 4.Department of Pathology, University of Chicago, Chicago, USA |
| 推荐引用方式 GB/T 7714 | Lee WH,Li Y,Wang W[*],et al. Variety of antimicrobial peptides in the Bombina maxima toad and evidence of their rapid diversification[J]. EUROPEAN JOURNAL OF IMMUNOLOGY,2005,35(4):1220-1229. |
| APA | Lee WH,Li Y,Wang W[*],Lai R,Li S,&Zhang Y[*].(2005).Variety of antimicrobial peptides in the Bombina maxima toad and evidence of their rapid diversification.EUROPEAN JOURNAL OF IMMUNOLOGY,35(4),1220-1229. |
| MLA | Lee WH,et al."Variety of antimicrobial peptides in the Bombina maxima toad and evidence of their rapid diversification".EUROPEAN JOURNAL OF IMMUNOLOGY 35.4(2005):1220-1229. |
入库方式： OAI收割
来源：昆明动物研究所
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