A Combinational Strategy upon RNA Sequencing and Peptidomics Unravels a Set of Novel Toxin Peptides in Scorpion Mesobuthus martensii
文献类型：期刊论文
| 作者 | Luan N1; Shen W1; Yang SL4,5,6; Lai R[*]1,4,5,6; Liu SQ[*]2,3; Rong MQ[*]4,5,6; Liu J2,3; Wen B2,3; Lin ZL2,3 |
| 刊名 | Toxins
 |
| 出版日期 | 2016 |
| 卷号 | 8期号:10页码:E286 |
| 关键词 | scorpion peptidomics toxins diversity |
| 通讯作者 | rlai72@njau.edu.cn ; rlai@mail.kiz.ac.cn ; siqiliu@genomics.cn ; rongmingqiang@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | Scorpion venom is deemed to contain many toxic peptides as an important source of natural compounds. Out of the two hundred proteins identified in Mesobuthus martensii (M. martensii), only a few peptide toxins have been found so far. Herein, a combinational approach based upon RNA sequencing and Liquid chromatography-mass spectrometry/mass spectrometry (LC MS/MS) was employed to explore the venom peptides in M. martensii. A total of 153 proteins were identified from the scorpion venom, 26 previously known and 127 newly identified. Of the novel toxins, 97 proteins exhibited sequence similarities to known toxins, and 30 were never reported. Combining peptidomic and transcriptomic analyses, the peptide sequence of BmKKx1 was reannotated and four disulfide bridges were confirmed within it. In light of the comparison of conservation and variety of toxin amino acid sequences, highly conserved and variable regions were perceived in 24 toxins that were parts of two sodium channel and two potassium channel toxins families. Taking all of this evidences together, the peptidomic analysis on M. martensii indeed identified numerous novel scorpion peptides, expanded our knowledge towards the venom diversity, and afforded a set of pharmaceutical candidates. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by NSFC (31260208, U1132601, 31200590, 81573320), CAS (SAJC201308, SAJC201606) and Yunnan Province (2011CI139, 2012BC009, P0120150010), Youth Innovation Promotion Association of CAS, Jiangsu Province (Q0201600440), the International Science & Technology Cooperation Program of China (2014DFB30020), Chinese National Basic Research Programs (2014CBA02002-A, 2014CBA02005) and the National High-Tech Research and Development Program of China (2012AA020202). |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/10611]  |
| 专题 | 昆明动物研究所_动物毒素室 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 |
| 作者单位 | 1.Life Sciences College of Nanjing Agricultural University, Nanjing 210095, Jiangsu, Chin 2.BGI-Shenzhen, Shenzhen 518083, China 3.China National GeneBank-Shenzhen, BGI-Shenzhen, Shenzhen, Guangdong 518083, Chin 4.Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming 650223, Yunnan, China 5.United Laboratory of Natural Peptide of University of Science and Technology of China & Kunming Institute of Zoology, Chinese Academy of Science, Kunming 650223, Yunnan, China 6.Sino-African Joint Research Center, Chinese Academy of Science, Wuhan 430074, Hubei, China |
| 推荐引用方式 GB/T 7714 | Luan N,Shen W,Yang SL,et al. A Combinational Strategy upon RNA Sequencing and Peptidomics Unravels a Set of Novel Toxin Peptides in Scorpion Mesobuthus martensii[J]. Toxins,2016,8(10):E286. |
| APA | Luan N.,Shen W.,Yang SL.,Lai R[*].,Liu SQ[*].,...&Lin ZL.(2016).A Combinational Strategy upon RNA Sequencing and Peptidomics Unravels a Set of Novel Toxin Peptides in Scorpion Mesobuthus martensii.Toxins,8(10),E286. |
| MLA | Luan N,et al."A Combinational Strategy upon RNA Sequencing and Peptidomics Unravels a Set of Novel Toxin Peptides in Scorpion Mesobuthus martensii".Toxins 8.10(2016):E286. |
入库方式： OAI收割
来源：昆明动物研究所
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