Jerdonase, a novel serine protease with kinin-releasing and fibrinogenolytic activity from Trimeresurus jerdonii venom
文献类型：期刊论文
| 作者 | Jia YH1,2; Jin Y1; Xiong YL[*]1; Lu QM[1,2]1; Li DS1; Wang WY1 |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2003 |
| 卷号 | 35期号:8页码:689-694 |
| 关键词 | fibrinogenase jerdonase kinin-releasing enzyme venom |
| 通讯作者 | wangwy@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | A novel kinin-releasing and fibrin (ogen)olytic enzyme termed jerdonase was purified to homogeneity from the venom of Trimeresurus jerdonii by DEAE Sephadex A-50 anion exchange, Sephadex G-100 (superfine) gel filtration and reverse-phase high performance liquid chromatography (RP-HPLC). Jerdonase migrated as a single band with an approximate molecular weight of 55 kD under the reduced conditions and 53 kD under the non-reduced conditions. The enzyme was a glycoprotein containing 35.8% neutral carbohydrate. The N-terminal amino acid sequence of jerdonase was determined to be IIGGDECNINEHPFLVALYDA, which showed high sequence identity to other snake venom serine proteases. Jerdonase catalyzed the hydrolysis of BAEE, S-2238 and S-2302, which was inhibited by phenymethylsulfonyl fluoride (PMSF), but not affected by ethylenediaminetetraacetic acid (EDTA). Jerdonase preferentially cleaved the Aalpha-chain of human fibrinogen with lower activity towards Bbeta-chain. Moreover, the enzyme hydrolyzed bovine low-molecular-mass kininogen and releasing bradykinin. In conclusion, all results indicated that jerdonase was a multifunctional venom serine protease. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/10622]  |
| 专题 | 昆明动物研究所_动物毒素室 昆明动物研究所_其他 |
| 作者单位 | 1.Chinese Acad Sci, Kunming Inst Zool, Dept Anim Toxicol, Kunming 650223, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jia YH,Jin Y,Xiong YL[*],et al. Jerdonase, a novel serine protease with kinin-releasing and fibrinogenolytic activity from Trimeresurus jerdonii venom[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2003,35(8):689-694. |
| APA | Jia YH,Jin Y,Xiong YL[*],Lu QM[1,2],Li DS,&Wang WY.(2003).Jerdonase, a novel serine protease with kinin-releasing and fibrinogenolytic activity from Trimeresurus jerdonii venom.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,35(8),689-694. |
| MLA | Jia YH,et al."Jerdonase, a novel serine protease with kinin-releasing and fibrinogenolytic activity from Trimeresurus jerdonii venom".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 35.8(2003):689-694. |
入库方式： OAI收割
来源：昆明动物研究所
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