Protease inhibitor in scorpion (Mesobuthus eupeus) venom prolongs the biological activities of the crude venom
文献类型：期刊论文
| 作者 | Hakim MA1,2; Tang XP1,2; Yang SL1,2; Lu QM[*]1,3; Lai R[*]1,3 |
| 刊名 | CHINESE JOURNAL OF NATURAL MEDICINES
 |
| 出版日期 | 2016 |
| 卷号 | 14期号:8页码:607-614 |
| 关键词 | Scorpion Mesobuthus eupeus Venom Protease inhibitor MeKTT-1 |
| 通讯作者 | rlai72@njau.edu.cn ; luqiumin@aliyun.com |
| 英文摘要 | It is hypothesized that protease inhibitors play an essential role in survival of venomous animals through protecting peptide/protein toxins from degradation by proteases in their prey or predators. However, the biological function of protease inhibitors in scorpion venoms remains unknown. In the present study, a trypsin inhibitor was purified and characterized from the venom of scorpion Mesobuthus eupeus, which enhanced the biological activities of crude venom components in mice when injected in combination with crude venom. This protease inhibitor, named MeKTT-1, belonged to Kunitz-type toxins subfamily. Native MeKTT-1 selectively inhibited trypsin with a K-i value of 130 nmol.L-1. Furthermore, MeKTT-1 was shown to be a thermo-stable peptide. In animal behavioral tests, MeKTT-1 prolonged the pain behavior induced by scorpion crude venom, suggesting that protease inhibitors in scorpion venom inhibited proteases and protect the functionally important peptide/protein toxins from degradation, consequently keeping them active longer. In conclusion, this was the first experimental evidence about the natural existence of serine protease inhibitor in the venom of scorpion Mesobuthus eupeus, which preserved the activity of venom components, suggests that scorpions may use protease inhibitors for survival. |
| 收录类别 | 其他 |
| 资助信息 | This work was supported by MOST (2013CB911300, 2010CB529800), NSFC (Nos. 31025025, 31025025, U1132601, 31200590), CAS (SAJC201308) and Yunnan Province (2011CI139, 2012BC009). |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/10765]  |
| 专题 | 昆明动物研究所_动物毒素室 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 |
| 作者单位 | 1.Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of sci- ences, Kunming 650223, China 2.Graduate University of Chinese Academy of Sciences, Beijing 100009, China 3.Joint Laboratory of Natural peptide, University of Science and Technology of China and Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China |
| 推荐引用方式 GB/T 7714 | Hakim MA,Tang XP,Yang SL,et al. Protease inhibitor in scorpion (Mesobuthus eupeus) venom prolongs the biological activities of the crude venom[J]. CHINESE JOURNAL OF NATURAL MEDICINES,2016,14(8):607-614. |
| APA | Hakim MA,Tang XP,Yang SL,Lu QM[*],&Lai R[*].(2016).Protease inhibitor in scorpion (Mesobuthus eupeus) venom prolongs the biological activities of the crude venom.CHINESE JOURNAL OF NATURAL MEDICINES,14(8),607-614. |
| MLA | Hakim MA,et al."Protease inhibitor in scorpion (Mesobuthus eupeus) venom prolongs the biological activities of the crude venom".CHINESE JOURNAL OF NATURAL MEDICINES 14.8(2016):607-614. |
入库方式： OAI收割
来源：昆明动物研究所
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