姚虻唾液腺中两种过敏原的结构和功能研究
文献类型：学位论文
| 作者 | 李元 |
| 学位类别 | 硕士 |
| 答辩日期 | 2011-05 |
| 授予单位 | 中国科学院研究生院 |
| 授予地点 | 北京 |
| 导师 | 赖仞 |
| 关键词 | 过敏原 吸血昆虫 牛虻唾液腺 抗原5蛋白 透明质酸酶 |
| 其他题名 | Studying on the structure and function of two allergens from salivary glands of the horsefly, Tabanus yao |
| 学位专业 | 生物化学与分子生物学 |
| 中文摘要 | 昆虫的刺蛰引起人体产生的过敏症是临床上关注的重要病症，除了能导致红肿和瘙痒等症状外，有时候全身过敏反应的发生甚至会导致患者的死亡。刺蛰的昆虫主要是膜翅目的胡蜂、黄蜂等，部分吸血类昆虫如蚊、蝇、牛虻等对人体的叮咬，也会导致过敏反应的发生。 牛虻在我国栖息地分布十分广阔，虻虫作为传统中药具有非常悠久的应用历史。但是牛虻的叮咬同时也能导致人体产生严重的过敏反应，而且暂时还未有牛虻过敏原的相关报道。 我们从姚虻唾液腺中分离纯化和鉴定了两种能与牛虻叮咬过敏病人血清中IgE特异性结合的蛋白Tab a 1和Tab a 2，分子量分别为26和35 kd，通过Edman降解法和cDNA克隆，我们获得了这两个蛋白的氨基酸序列。通过酶联免疫吸附测定（ELISA），ELISA抑制实验和Western Blotting确定了它们的过敏原性。 通过氨基酸序列分析，确定Tab a 1为一种在昆虫中常见的过敏原Ag 5蛋白类似物，Tab a 2为透明质酸酶类似物。Western Blotting的结果显示Tab a 1和Tab a 2分别可以37份过敏原病人血清中32份和34份（86.5%和91.8%）的IgE特异性结合。ELISA抑制试验显示Tab a 1和Tab a 2都能显著地抑制牛虻唾液腺提取物与过敏病人血清中IgE的特异性结合(抑制率均可达到45%左右)，另外这两种蛋白分子还能与胡蜂刺蛰后过敏病人血清中的IgE特异性结合。 这是首次从牛虻唾液腺中鉴定出过敏原蛋白的报道。实验表明Tab a 1和Tab a 2在牛虻叮咬致敏反应中发挥重要作用，同时Tab a 1与Tab a 2均能与胡蜂刺蛰后过敏病人血清中的IgE特异性结合，抑制其与胡蜂毒液的结合，这一结果为医学上推测的胡蜂—牛虻交叉过敏综合症提供了有力的证据。该工作为牛虻唾液腺中过敏原的进一步分析奠定了基础，同时也为研究吸血节肢动物的过敏原提供了思路，并为牛虻的生物防治提供了思路和对策。 |
| 英文摘要 | Anaphylaxis caused by insects stings is an important clinical disease, it may induce a local wheal and pruritus response. However, many cases of insects allergy with severe allergic reactions even death have been recorded. Allergy from insect venom is mostly caused by Hymenoptera stings, such as wasps and hornets, but has been also reported after bites by various blood-feeding arthropods such as mosquitoes, flies and horseflies. Horseflies are mainly distributed in pastoral area, and they have been used as a traditional Chinese medecine for thousands of year. Horsefly bite can cause severe allergic reactions in humans. There is no information about allergenic horsefly proteins. Two IgE-binding proteins, Tab a 1 and Tab a 2 with molecular weight of 26 and 35 kd, respectively, were purified and characterized from horsefly salivary glands of Tabanus yao. Their primary sequences were determined by Edman degradation and cDNA cloning. Their allergenicity was examined using enzyme-linked immunosorbent assay (ELISA) , ELISA inhibition tests and immunoblots. They were identified as an antigen 5-related (Ag 5) protein and hyaluronidase, respectively. Immunoblotting demonstrated IgE binding by 32 and 34 of 37 (86.5% and 91.8% ) subjects’ sera to Tab a 1 and Tab a 2, respectively. ELISA inhibitions of serum IgE reactivity to the horsefly salivary gland extract (SGE) using purified Tab a 1 and Tab a 2 were significant (about 45% ). In addition, these proteins showed some IgE-binding capacity to sera of subjects with wasp sting allergy. We have first identified and characterized two IgE-binding proteins from horsefly salivary gland. They appear to be of importance for the allergic reactions induced by horsefly bite. These allergens are thus not only found in stinging but also found in hematophagous insects. These results provided support for the presence of the so-called wasp-horsefly syndrome (WHS ). It is possible that there are other allergens in the horsefly saliva. To develop highly sensitive immunoassays for the diagnosis of horsefly allergy and effectively treat the individual who is sensitized by the particular allergens, further work is necessary to identify and characterize all of the allergens in the horsefly saliva. |
| 语种 | 中文 |
| 公开日期 | 2011-09-21 |
| 源URL | [http://159.226.149.42:8088/handle/353002/6800]  |
| 专题 | 昆明动物研究所_动物毒素室 |
| 推荐引用方式 GB/T 7714 | 李元. 姚虻唾液腺中两种过敏原的结构和功能研究[D]. 北京. 中国科学院研究生院. 2011. |
入库方式： OAI收割
来源：昆明动物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。